You are studying the binding of proteins to thecytoplasmic face of cultured neuroblastoma cells andhave found a method that gives a good yield of inside-outvesicles from the plasma membrane. Unfortunately, yourpreparations are contaminated with variable amounts ofright-side-out vesicles. Nothing you have tried avoids thisproblem. A friend suggests that you pass your vesicles overan affinity column made of lectin coupled to solid beads.What is the point of your friend’s suggestion?

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Osmosis

In simple words, osmosis is the slow, gradual movement of a liquid through a membrane. But there are nuances to this process. For instance, there has to be an imbalance in the distribution of solute in the liquid on either side of the membrane, which has to be semipermeable. The liquid in the region with a lower solute concentration moves to the region with a higher concentration, thereby displacing the solution in the process. The objective is to equalize the concentration of molecules on both sides of the membrane; the displacement of liquid can be viewed as a byproduct of this equalization. Since there is no energy required to carry osmosis, it is considered a passive process.