You are studying how a bacteria grows in various buffers, and also the impact osmo-pressure has on its survival A. The bacteria has [solute] conc. 150 nM. Out of the first 3 buffers (1-3), which one is hypertonic, isotonic and hypotonic environments. Explain reasoning. B. You attempt to grow the bacteria in coffee but there isnt bacteria growth and it actually lyses. Is coffe environment hypertonic, isotonic, or hypotonic, explain reasoning. And what [coffee] conc. causes it to lyse. C. You use a different bacteria and grow it in Buffer 3, and it is isotonic. What about the other buffers, will it be isotonic, hypertonic, or hypotonic

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