You are making a potassium phosphate buffer that has 54 mM total phosphate (weighed out as KH2PO4), 205 mM KCI, and 7 mM dithiothreitol. You need 299 ml of buffer, which will be titrated to pH 54. What volume of a 25 mM dithiothreitol stock solution should you add to the flask before diluting it to the final volume for your buffer? Report the volume in ml to the nearest 1 mL.

You are making a potassium phosphate buffer that has 54 mM total phosphate (weighed out as KH2PO4), 205 mM KCI, and 7 mM dithiothreitol. You need 299 ml of buffer, which will be titrated to pH 54. What volume of a 25 mM dithiothreitol stock solution should you add to the flask before diluting it to the final volume for your buffer? Report the volume in ml to the nearest 1 mL.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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