You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts 3)You seed another culture of a different end cell line, john12 cells at 8am on Friday with 104 cells. By Thursday at noon (12:00pm) they reach confluence. Assume the same confluency as stated above for T150 flask. What is the generation time (doubling time)
You are growing up myoblasts, C2C12 cells, to use in a myogenic study. You are using T-150 flasks with a culture area of 150 cm2 and when confluent contains 2 x 107 cells.You are growing up myoblasts
3)You seed another culture of a different end cell line, john12 cells at 8am on Friday with 104 cells. By Thursday at noon (12:00pm) they reach confluence. Assume the same confluency as stated above for T150 flask. What is the generation time (doubling time)
Cell division is the process by which the cells grow and give rise to new daughter cells. Doubling time is referred to the time taken by the cells to divide.
Confluency is reached when the entire surface area of the instrument in which the cells are growing is occupied by the cells and there is no gap present between the cells.
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