You are designing a drug that will harm bacteria but leave eukaryotic cells alone. To accomplish this Would you design a that drug that works by damaging the plasma membrane? Explain. (This is a microbiology q)
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- Please I don’t understand this Biochem question. An explanation leading to both of the CORRECT answers would be helpful!The most selectively toxic antibacterial agents are those that interfere with bacterial cell wall synthesis. This is because ________. Group of answer choices bacterial cell wall synthesis is easily inhibited whereas eukaryotic cell wall synthesis is more resistant to the actions of the drugs bacterial cell walls have a unique structure not found in eukaryotic host cells eukaryotic cells do not take up the drugs eukaryotic cells inactivate the drugs before they can do any damageTRY TO KEEP IN SHORT AND USE OWN WORD FOR THIS QUESTION You are studying a type of bacteria isolated from the acidic water runoff of a mining operation. You subject two batches of the same bacteria type to different environmental growth conditions. One batch is grown at pH 2, while the other is grown at pH 7. All other environmental parameters are kept identical between the two batches. You then collect their proteins and run a Western blot using an antibody that binds to a proton efflux pump protein (which actively expends energy to pump protons out of a cell). How would you characterize the information obtained in this experiment? What does it tell you, and why is that potentially valuable information?
- Most antibacterial drugs disrupt or destroy prokaryotic cellular structures or processes that are different than those of eukaryotic cells or may not even be present in eukaryotic cells. List and describe at least three prokaryotic cellular features that could be targeted to inhibit or kill a bacterial pathogen without harming its eukaryotic host.Spectrum of activity describes how many species of microbes a drug works against. Broad-spectrum antibiotics are effective against many different groups of bacteria, while narrow-spectrum antibiotics work well against a particular group or species, but have little effect on the rest. Based on our class results, most of the narrow-spectrum antibiotics we tested target what? Group of answer choices DNA replication/synthesis Essential metabolite synthesis Protein synthesis Plasma membrane Cell wall formation OtherThe following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.
- You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions: a) Which technique will you use to isolate mitochondria? b) What happens to the pH of the medium when the medium is kept anaerobic? c) What happens when O2-saturated saline is added to the mixture?Then there are the drugs that inhibit metabolic pathways. Describe how sulfa drugs act to prevent the bacterial cell from producing folic acid which is necessary for DNA production and thus binary fission.Why are bacterial DNA gyrase and topoisomerase good targets for antimicrobial drugs? These enzymes are not necessary for DNA replication. These enzymes are involved in energy production, so targeting them would prevent the bacteria from obtaining necessary energy. These enzymes are different from their eukaryotic counterparts, so the drugs targeting them should not affect the host. These enzymes are responsible for host cell recognition, so targeting them would prevent the host cell from being infected.Flag this QuestionQuestion 10
- Spectrum of activity describes how many species of microbes a drug works against. Broad-spectrum antibiotics are effective against many different groups of bacteria, while narrow-spectrum antibiotics work well against a particular group or species, but have little effect on the rest. Based on our class results, which of the following are narrow-spectrum? Group of answer choices Amoxicillin/Clavulanic acid Ampicillin Kanamycin Ciprofloxacin Vancomycin Bacitracin Trimethoprim/SulfamethoxazoleChoose the false statement: Penicillin is a ß lactam which inhibits bacterial growth by inhibiting DNA polymerase. O Resistance to ß lactamase inhibitors arises when bacteria duplicate the ß lactamase gene. O B lactamase inhibitors are prescribed together with antibiotics like penicillin in order to combat resistance to these antibiotics. Question 10 Choose the false statement below: OUsing multiple antimicrobials in combination is an ineffective strategy because they generally are redundant in effect and the chance of drug resistance will be increased. O Because of its complex structure and multiple binding sites, the ribosome is the target for multiple types of antibiotics. O Choice of an antimicrobial agent may be dependent upon the material treated as well as the susceptíbility of the microbe.In terms of microbial pathogenicity, molecular Koch's postulates are a set of experimental criteria that show: A microbe is the aetiological agent of a disease A gene encodes a product that contributes to disease A gene encodes a product that contributes to immunity to a disease A mutation does not contribute to disease