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- Making ATP Directions: Compare substrate level phosphorylation and oxidative phosphorylation by completing this table. Descriptions that are questions are yes/no questions. Description Location(s) in the cell (be specific) Requires a proton gradient? Uses chemiosmosis? Requires a soluble enzyme (an enzyme in solution, not in a membrane)? Requires a transmembrane protein? Occurs during glycolysis? Occurs during pyruvate oxidation? Occurs during citric acid cycle? Requires the electron transport chain? Approximate number of ATP made per 1 glucose in aerobic respiration Number of ATP made per 1 glucose in fermentation Substrate level phosphorylation Oxidative phosphorylationSaccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP Two moles of fructose-1,6-biphosphateSaccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP 1. Three moles of glucose-6-phosphate 2. Four moles of pyruvic acid
- Saccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP 1. Three moles of glucose-6-phosphateSaccharides: Using the following substrates, estimate the net ATP yield after glycolytic pathway, Kreb’s cycle and electron transport chain. Assume that the estimate for ATP yield per mole of NADH is 3 moles of ATP, while 1 mole of FADH2 is equivalent to 2 moles of ATP, and one mole of GTP is equivalent to one mole of ATP. Show all pertinent solutions and determine: a) ATP used, b) ATP produced, and c) Net ATP. Based on your solutions, rank the substrates based on increasing yield of ATP Five moles of Acetyl coenzyme ARemember for T/F questions, either answer TRUE or FALSE, but if the answer is FALSE make sure to explain WHY the answer is false. The cell prefers lipids as a food source due to the large amount of NADH it can create during fatty acid oxidation is then used to create ATP. 1 A- Ff▼ = = F B I U S X₂ x² % S'S ↓ S 8- OiLearn Video
- Bioenergetics: Answer the following questions regarding bioenergetics/ oxidative phosphorylation: Given the following reactions and AGs for each reaction, answer the two questions that follow; be sure to give complete answer including proper units and sign(s) wherever necessary: Reaction #1 (AG = -2.3 kcal/mol): A+ B2 C+D Reaction #2 (AG = +5.3 kcal/mol): C+ K 2R Reaction #3 (AG = -4.5 kcal/mol): R 2 Y+Z 7. Calculate AG for overall reaction: AR>Z Free Energy Change Reactions #1-3 = 8. Which direction is this overall process (#1-3) predicted to go (circle one)? forward OR backwardLocalization of Molecules and Functions Within the Mitochondrion. Indicate whether you would expect to find each of the following molecules or functions in the matrix (MA), the inner membrane (IM), the outer membrane (OM), the intermembrane space (IS), or not in the mitochondrion at all (NO). Write abbreviation in front of the molecules. No explanation is required. Succinate dehydrogenase Coenzyme A Conversion of lactate into pyruvate ATP synthase Malate dehydrogenase Pyruvate Citrate synthase Cristae Accumulation of a high proton concentration Pyruvate dehydrogenaseThe following structure is ATP synthase. Which of the following statements is NOT true for the ATP synthase? B b2 6 a В a Y E H+ α C10 B ATP ADP + Pi N side 800000 P side
- Matching (Letters may be used twice) DD DDD DD Where is the electron transport chain located? Where does the Krebs cycle occur? What membrane do protons cross in the synthesis of ATP? Where does glycolysis occur? Where does the oxidation of pyruvate occur? Where does the most substrate level phosphorylation occur? Where is most NADH oxidized to NAD+? What membrane does glucose have to cross? 1. Cell membrane 2. nucleus 3. intermembrane space 4. mitochondrial matrix 5. chloroplast 6. cytoplasm 7. outer mitochondrial membrane 8. inner mitochondrial membraneIn full details. Explain the significance of redox potentials formed by redox pairs in the electron transport chain.Additional information: ATP production by the ETC and ATP Synthase per glucose varies somewhat depending on the energy required to move NADH into the mitochondria and other energy uses for the hydrogen-ion gradient. Additional questions: 1.) How many ATP's are generally yielded by oxidative phosphorylation from the catabolism of one glucose? 2.) Summarize the total ATP's obtained from a single molecule of glucose, from start to finish? asap