Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Write a conclusion based on the result above

Transcribed Image Text:Standard Tubes
Volume of 0.1
Hydroxamate
standard
Optical Density
(OD)
Tubes
Concentration
0.061
0.078
1
0.1
0.002
3
0.2
0.095
0.004
4
0.4
0.125
0.008
Standard Curve of Optical Density (OD) vs Concentration of
Hydroxamate
0.14
0.12
0.1
0.08
y= 7.9857x +0.0618
R = 0.9988
0.06
0.04
D.02
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0,009
Concentration of Hydroxamate
Figure 1: Graph of Standard Curve of Optical Density against the Concentration of
Hydroxamate.
Hydroxamate Assay Tubes
From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the
equation, y intercept was the optical density and x-intercept was the concentration of
hydroxamate.
Optical Density at 520 nm
Concentration of
hydroxamate formed
Tubes
5
0.103
0.0052
0.077
0.0019
7
0.123
0.0077
0.135
0.114
0.065
0.0092
0.0065
10
0.0004
Optical Density at 520 nm
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