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Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Standard Tubes Volume of 0.1 Hydroxamate standard Optical Density (OD) Tubes Concentration 0.061 0.078 1 0.1 0.002 3 0.2 0.095 0.004 4 0.4 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 0.08 y= 7.9857x +0.0618 R = 0.9988 0.06 0.04 D.02 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0,009 Concentration of Hydroxamate Figure 1: Graph of Standard Curve of Optical Density against the Concentration of Hydroxamate. Hydroxamate Assay Tubes From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the equation, y intercept was the optical density and x-intercept was the concentration of hydroxamate. Optical Density at 520 nm Concentration of hydroxamate formed Tubes 5 0.103 0.0052 0.077 0.0019 7 0.123 0.0077 0.135 0.114 0.065 0.0092 0.0065 10 0.0004 Optical Density at 520 nm
Transcribed Image Text:Standard Tubes Volume of 0.1 Hydroxamate standard Optical Density (OD) Tubes Concentration 0.061 0.078 1 0.1 0.002 3 0.2 0.095 0.004 4 0.4 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 0.08 y= 7.9857x +0.0618 R = 0.9988 0.06 0.04 D.02 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0,009 Concentration of Hydroxamate Figure 1: Graph of Standard Curve of Optical Density against the Concentration of Hydroxamate. Hydroxamate Assay Tubes From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the equation, y intercept was the optical density and x-intercept was the concentration of hydroxamate. Optical Density at 520 nm Concentration of hydroxamate formed Tubes 5 0.103 0.0052 0.077 0.0019 7 0.123 0.0077 0.135 0.114 0.065 0.0092 0.0065 10 0.0004 Optical Density at 520 nm
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