Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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5.

Would it be possible to start synthesizing the daughter DNA strand
without assembling the RNA primer first? Why? Why not?
Transcribed Image Text:Would it be possible to start synthesizing the daughter DNA strand without assembling the RNA primer first? Why? Why not?
A. No, because the RNA primer which contains the free 3' OH in its
ribose has to be synthesized by primase first.
B. Yes, because the 3' OH is already present in the DNA strand which
will be used as a template.
c. Yes, because the 5' PO4 is already present in the DNA strand
which will be used as a template.
D.No, because the RNA primer which contains the free 5' PO4 in its
ribose will not be synthesized by primase.
Transcribed Image Text:A. No, because the RNA primer which contains the free 3' OH in its ribose has to be synthesized by primase first. B. Yes, because the 3' OH is already present in the DNA strand which will be used as a template. c. Yes, because the 5' PO4 is already present in the DNA strand which will be used as a template. D.No, because the RNA primer which contains the free 5' PO4 in its ribose will not be synthesized by primase.
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