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Why would slants stored at different temperatures look the same?
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- Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to measure your samples? Absorbance 400 500 600 Wavelength (nm) 700How are these equal? How would I know to match these? 25 km = 25,000 m 2.5 cm = 2.5 × 104 μm 0.25 m = 2.5 × 108 nm 2.5 mm = 2.5 × 10-6 kmWhy do some materials exhibit fluorescence when exposed to ultraviolet light?
- (b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…why steroids do not show luminescence on thin layer chromatographic plates?Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured with the spectrophotometer to have an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? 0.9 0.8 0.7 0.6 805 4 0.4 0.3 0.2 Absorbance at 540 m
- Discuss the types of interferences in atomic absorption spectrophotometry.In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.Besides 1H NMR, FT-IR, melting point, data for refractive index, what other information could you obtain for your compound to assess its’ purity and confirm its’ structure? How would this additional data contribute to your conclusion about the structure of the unknown compound?
- What is an absorption spectrum? The following graph is the visible absorbance spectrum of an indicatorsolution. How can we conclude about the color of this sample?what does it mean if a pigment (let' say 'yellow') traveled beyond the solvent front in paper chromatography?Why does one see methyl orange as orange in color? What blank would be used to standardize this spectrophotometer?
