Q: How is the India ink capsule stain an example of a negative stain?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: why are the steps in gram staining carefully standardized?
A: Gram staining The technique of differentiation of two large group of bacteria that is gram positive…
Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…
A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…
Q: Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
A: The growth media are nutritious media that are used to grow microbes (bacteria and fungus) in the…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.
A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…
Q: why are the steps in gram staining are so carefully standardized?
A: Gram staining is very useful process that differentiate bacteria on the basis of their cell wall…
Q: two different
A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…
Q: Kari and Jamal are studying a genetic trait that is controlled by two genes, Gene 1 and Gene 2. Kari…
A: In molecular biology there is a theory called central dogma of life. This theory states that the…
Q: Why is it essential that the primary stain and the counterstain be contrasting colors in…
A: The gram staining is used to distinguish between gram negative and gram positive bacteria. It was…
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: Is there a relationship between Gram staining and sensitivity to various antibacterial compounds?…
A: Gram staining is a method to identify whether a bacteria is gram positive or gram negative based on…
Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).
A: The danish Bacteriologist Christian gram discovered this differential technique in order to…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Define the term gram staining.
A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…
Q: This part of the staining process helps the primary stain to remain in the cell during…
A: Staining of biological samples is a very useful process for detailed observation and measurement of…
Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…
A: The differential stain is used to distinguish between different structures and cellular components.…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: what would be the medical significance of finding a positive endospore stain result from bacilli…
A: Endospores are thick walled structures found in the genus Bacillus and Clostridium.
Q: Why can’t gram staining be used for mycobacterium?
A: Mycobacteria are acid fast bacteria which contains mycolic acid in their outer membrane, making…
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: You have performed an experiment and your aim is to obtain pure colonies. However, you observe some…
A: Bacteria are prokaryotic organisms. They are microscopic single-celled organisms that can live in a…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of
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- What is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedYou have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?Why can’t gram staining be used for mycobacterium?
- why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.Which step is the most crucial or most likely to cause poor results in the Gram stain?
- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantwhat would be the medical significance of finding a positive endospore stain result from bacilli bacteria obtained from a sample of honey that was fed to a baby wtih severe gastrointestinal distress?