Why is it necessary to chelate the metal ions from the solution during the boiling/lysis step at 100 degrees celsius? What would happen if you did not use a chelating agent such as the InstaGene matrix? What is needed from the cells for PCR? What structures must be broken to release the DNA from a cell?

Q: Gel electrophoresis can be used for all of the following purposes except. (A) Determining the…

A: Gel electrophoresis is the technique of separating macromolecules like DNA, RNA and protein with the…

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: What is the size of the PCR product (Hse gene) observed from the gel photo?

A: ** The expected size of PCR product is 357+280bp, or 637np.                                     **…

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…

A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…

Q: a. Explain whether or not DNA polymerase from a mesophilic bacterium could be used successfully in a…

A: A)- In a mesophile optimum condition would allow the microbe to replicate at it maximum amount…

Q: The synthesis of phosphocreatine is unfavorable. If dur- ng extended periods of rest, e.g., sleeping…

A: Phosphocreatine (PCr) -  It is a phosphorylated creatine molecule that works as transferable reserve…

Q: Sort the steps to be followed in order to produce human growth hormone (HGH) in the corre Extract…

A: Creating human growth hormone (HGH) using genetic engineering is like a science fiction adventure in…

Q: List and briefly describe the function of 4 proteins used in DNA replication in a E. coli but NOT…

A: The process of copying a DNA molecule to produce its two identical copies is termed as the DNA…

Q: This enzyme will be added when the cells are lysed, detroying all RNA in the sample. Why will this…

A: INTRODUCTION Plasmid Plasmid are circular DNA and into which we can insert the DNA of our interest…

Q: PCR has revolutionalized biotechnology. Discuss three advantages and three disadvantages of PCR…

A: PCR or Polymerase Chain Reaction is the technique which is used for making many copies of a specific…

Q: Why is it important to sequence positive clones derived from PCR cloning? Group of answer choices

A: PCR is a technique of amplification of a given molecule of DNA to increase its copy number.

Q: Coomassie dye is the equivalent of what component used in analysis of DNA by agarose gel…

A: Western blot helps to identify specific proteins from a mixture of proteins and this step follows…

Q: Why uncut and cut PCR at different position in the agarose gel? which one can be decided accuracy…

A: *Gel electrophoresis a technique to separate DNA fragments based on their size. *DNA samples are…

Q: 1. Explain what primers are and what purpose they serve in a PCR reaction. Explain the main steps…

A: “Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: Cause Would result in the following error Unbalanced nucleotide concentrations a) Sequence errors in…

A: The polymerase chain reaction is the method that is used for making copies of DNA, it is developed…

Q: Explain how and why PCR can be used to amplify DNA. Describe the steps in the process. Be sure to…

A: PCR stands for a polymerase chain reaction. It is used to amplify the DNA segment. It can make many…

Q: Briefly Explain the role of restriction enzymes in recombinant DNA technology. Please explain at…

A: DNA is a type of nucleic acid that is available in the nucleus of the cell. It is the chemical…

Q: In PCR amplification why is it important to have a negative control?

A: Introduction :- The Polymerase Chain Reaction (PCR), which is based on the idea of enzymatic…

Q: please answer these questions for this image What is the source of the DNA? What is the target?…

A: The objective of the question is to understand the process of genetic engineering, specifically the…

Q: What type of enzymes are used to “cut” desired DNA sequences for use in recombinant gene technology…

A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…

Q: Briefly explain how the polymerase chain reaction is used to amplify a specific DNA sequence. What…

A: PCR which stands for polymerization chain reaction is a rapid and extremely sensitive analytical…

Q: What is the role of streptomycin in CRISPR experiment? What biochemical changes (DNA, protein)…

A: The genetic tool CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has…

Q: Microarray hybridization is used mostly in transcript profiling or assaying DNA variation.  Although…

A: A usual microarray technology includes the hybridization of an mRNA with its original template of…

Q: Briefly describe what happens during each of the phases of PCR (denaturation, annealing, and…

A: The “polymerase chain reaction” (PCR) is a technique in which millions of copies of the target DNA…

Q: In a PCR assay, what's the purpose of each temperature step at: -heating the sample to 50 C…

A: PCR (polymerase chain reaction) is a technique for analysing a short sequence of DNA (or RNA) also…