Why does in this experiment of invertase subjecting it to benedicts tests shows that adding 3ml of yeast from the sample LOT A shows yellow color while adding another water on the other test tube does not changes its color, what is the reason behind it?
Organic Chemistry of Metabolic Pathways
Metabolic pathways allude to the arrangement of chemical catalyzed reactions that lead to the transformation of a substance into the final product. Metabolic pathways incorporate a progression of reaction where the substrate is changed continuously and the transitional metabolites are persistently recovered.
Glucogenesis
Glucogenesis is a metabolic pathway in which glucose is produced from carbon substrates that are not carbohydrates. This process is observed in plants, animals, fungi, bacteria and other micro organisms. The general definition for glucogenesis or gluconeogenesis is as follows,
Why does in this experiment of invertase subjecting it to benedicts tests shows that adding 3ml of yeast from the sample LOT A shows yellow color while adding another water on the other test tube does not changes its color, what is the reason behind it?
![1.5 INVERTASE
OBJECTIVES:
> To know the characteristics of invertase
> To enumerate the functions of invertase
> To explain the principle of invertase test
MATERIALS:
Sucrose (0.25M solution) Benedicts solution
Test Tubes Bunsen burner and tripod set up
Distilled water Stiring rods
Beakers Pipettes
Dry yeast Dropper
PROCEDURE:
1. Prepare yeast by mixing 3 grams of dry yeast with 20 ml of distilled water. Let stand for 20 minutes.
2. Fill each of two test tubes 1/3 full with sucrose solution.
3. Add 3 ml yeast suspension to one tube (Lot A). Mix.
4. Add 3 ml distilled water to the other tube (Lot B). Mix.
5. After 10 minutes, test each test tube with Benedicts solution. Remove about 2 ml of the solution,
place in another test tube, add 10 drops of Benedicts solution and place in boiling water bath for
about 3 minutes. Note color changes/precipitation.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa69fcad6-82c1-43f4-82c8-a49e38276de4%2F1476632f-361a-4f19-879f-1d1375acaf41%2Ft66bmk6_processed.png&w=3840&q=75)
![DATA SHEET
SAMPLE
BEFORE
AFTER
LOT A
LOT B](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa69fcad6-82c1-43f4-82c8-a49e38276de4%2F1476632f-361a-4f19-879f-1d1375acaf41%2Fqgepv8n_processed.png&w=3840&q=75)
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