Why does in this experiment of invertase subjecting it to benedicts tests shows that adding 3ml of yeast from the sample LOT A shows yellow color while adding another water on the other test tube does not changes its color, what is the reason behind it?

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Why does in this experiment of invertase subjecting it to benedicts tests shows that adding 3ml of yeast from the sample LOT A shows yellow color while adding another water on the other test tube does not changes its color, what is the reason behind it?

1.5 INVERTASE
OBJECTIVES:
> To know the characteristics of invertase
> To enumerate the functions of invertase
> To explain the principle of invertase test
MATERIALS:
Sucrose (0.25M solution) Benedicts solution
Test Tubes Bunsen burner and tripod set up
Distilled water Stiring rods
Beakers Pipettes
Dry yeast Dropper
PROCEDURE:
1. Prepare yeast by mixing 3 grams of dry yeast with 20 ml of distilled water. Let stand for 20 minutes.
2. Fill each of two test tubes 1/3 full with sucrose solution.
3. Add 3 ml yeast suspension to one tube (Lot A). Mix.
4. Add 3 ml distilled water to the other tube (Lot B). Mix.
5. After 10 minutes, test each test tube with Benedicts solution. Remove about 2 ml of the solution,
place in another test tube, add 10 drops of Benedicts solution and place in boiling water bath for
about 3 minutes. Note color changes/precipitation.
Transcribed Image Text:1.5 INVERTASE OBJECTIVES: > To know the characteristics of invertase > To enumerate the functions of invertase > To explain the principle of invertase test MATERIALS: Sucrose (0.25M solution) Benedicts solution Test Tubes Bunsen burner and tripod set up Distilled water Stiring rods Beakers Pipettes Dry yeast Dropper PROCEDURE: 1. Prepare yeast by mixing 3 grams of dry yeast with 20 ml of distilled water. Let stand for 20 minutes. 2. Fill each of two test tubes 1/3 full with sucrose solution. 3. Add 3 ml yeast suspension to one tube (Lot A). Mix. 4. Add 3 ml distilled water to the other tube (Lot B). Mix. 5. After 10 minutes, test each test tube with Benedicts solution. Remove about 2 ml of the solution, place in another test tube, add 10 drops of Benedicts solution and place in boiling water bath for about 3 minutes. Note color changes/precipitation.
DATA SHEET
SAMPLE
BEFORE
AFTER
LOT A
LOT B
Transcribed Image Text:DATA SHEET SAMPLE BEFORE AFTER LOT A LOT B
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