Why do you have to reduce the amount of light with the diaphragm in order to see bacteria in a hanging drop slide? What is the value of a hanging-drop preparation? Wet-mount preparation?
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- If we used the 365nm setting on the UV light box would this have caused more or less UV light damage, explain why?What is the significance of using oil with the immersion lens and why can't oil be used with any other lens?Which of the following will decrease the limit of resolution (Select all that applies) a) using lower wavelength of illumination b) using an objective lens with lower numerical aperature c) using immersion oil d) using electron as the source of illumination instead of light
- Why do you use immersion oil with 100X objective lens?If we used the 365nm setting on the UV light box would this have caused more or less UV light damageWhile looking into your compound light microscope you find that only part of the specimen is visible. What might be the cause? Question options: Insufficient illumination from the substage condenser. Iris diaphragm has not been properly adjusted. Your objective lens has not clicked fully into position. Your objective lens is dirty.
- Use the iris diaphragm to attain the best illumination of the letter “e” on the scanning (4x) objective. Open and close the iris diaphragm and notice the changes in contrast of the letter “e”. How did the appearance of the letter “e” change under the various levels of illumination? 1 Repeat the above procedure on the high-power (40x) objective. When increasing magnification, what happens to the light available? Why?How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!If a microscope ocular lens has a power of 10x and the objective lens has a power of 20x then the overall magnification is: 20 12 30 120 200
- What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to absorb light in a spectrophotometer at a UV range or at a colorless wavelength? Please provide detailed and clear responses. Thank you very much!How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityD) When performing fluorescence microscopy what are the stokes shift and why is it better to have fluorochromes with a large stokes shift? E) What is photobleaching and what is done when imaging histological samples to overcome it when performing fluorescence microscopy?
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