riefly describe the functions of the following parts of the microscope: course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.
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briefly describe the functions of the following parts of the microscope:
course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.
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- How will the following affect resolution during microscopy? I) Closing or opening the diaphragm II) Raising or lowering the condenser III) Increasing or reducing the light intensityComplete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30
- Label the parts of the microscope on the diagram below: arm, stage, base, ocular, revolving nosepiece, objective (4x, 10x, 40x), course focus, fine focus, condenser & iris diaphragm (these are marked by the same line)Total magnification of a microscope is determined by Multiple Choice dividing the objective magnification value by the ocular value. multiplying all objective magnification values together. adding all objective magnification values together. none of the above.If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400x
- When focusing a light microscope, why is it best to adjust the focus using the coarse focusing knob before using the fine focusing knob?Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the center
- The objective lenses of the microscopes used in this course are all parfocal. What does having parfocal objective lenses mean? Question options: The slide has to be moved away from the objective lens (i.e., lowered) when switching between objective lenses. None of these choices The objective lenses have optimized light gathering capability. Once in focus, you may switch between objective lenses with only fine focus adjustment required.Please answer the following questions (Question 1 AND 2) about compound microscopes. After clicking the objective into position, which adjustment knob(s) can you use to safely refocus the image? You may need to adjust the light intensity to better see the specimen. Indicate two ways in which you can change the light intensity?Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?