Why do we use turbidity as a method to measure bacterial growth? How does it work? How do you interpret the theoretical and actual results of turbidity measurements? Can you think of advantages and disadvantages to using this type of method?

Q: 1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B)…

A: A spectrophotometer is an instrument that measures the number of photons (light intensity) absorbed…

Q: Explain growth-cycle phases for a batch cultivation. Does Penicillin production occur in log or…

A: Germs need certain conditions in order to grow, and these conditions are not unique to all bacteria.…

Q: (1) What is the difference between specific growth rate and generation time?

A: As per our policy we are answering the first question only.  For the rest of the question kindly…

Q: Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain…

A: SYBR Green (SG) is an asymmetrical cyanine nucleic acid stain. It binds to DNA.

Q: Consider the growth curve shown below. Cells were grown in a medium containing 1 % w/v glucose and…

A: The given graph represent the biphasic growth of cells. Cells are grown on two carbon sources…

Q: In what ways does the neural control of smooth muscle activitydiffer from that of skeletal muscle?

A: Muscles are contractile tissues grouped and coordinated in function. Three groups of muscles present…

Q: You are examining a growth curve data from an experiment. At time 70 min, the optical density was…

A: Bacteria grow very fast if the get the proper medium for their growth and the generation time is the…

Q: Summarize the different levels of gene regulation ineukaryotes.

A: Gene regulation is the way toward controlling which genes in a cell's DNA are communicated (used to…

Q: In zone electrophoresis, why is it possible to isolate the components of a sample and recover them…

A: Introduction :- Electrophoresis is a laboratory technique used to separate and analyze DNA, RNA,…

Q: What is the best growth medium that contains glucose that can be used to acclimatize bacteria? and…

A: A growth medium is a liquid, solid, or semi-solid preparation that supports the growth of a…

Q: what is the hydrodynamic stress of bioreactors when there are cellscultures?

A: Hydrodynamic stress is defined as the pressure exerted due to motion of fluid as water. Hydrodynamic…

Q: Why is it necessary to make pure subcultures of organisms grown from clinical specimens? How does…

A: Introduction: A subculture is developed by transferring several cells from an earlier culture to a…

Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…

A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…

Q: What is the application of Gel Electrophoresis in healthcare? Cite at least 2 specific examples.

A: Gel Electrophoresis: A laboratory technique called gel electrophoresis is employed to divide…

Q: A culture medium contains 1mM NaCl, 1mM of each essential amino acid, and 5g/L honey. Is the medium…

A: A culture medium is defined if it contains various constituents with known and measurable…

Q: Bacillus subtilis is known to harbour proA gene which is expressed as protease. Protease is an…

A: Genetic engineering and bioprocess are very good tools in the pharmaceutical, therapeutics, enzyme…

Q: What is the purpose of the baking soda solution in the leaf disks experiment?

A: Leaf disks experiment is an experimental set up which is aimed at studying the effects of various…

Q: Differential centrifugation can be improved through the use of a density gradient. What is the…

A: In scientific experiments, various techniques are used to isolate, measure or quantify samples based…

Q: What is the best descriptive match for the following culture media? A. Specific biological…

A: Question no 1 Synthetic media: In this type of medium all the elements/chemicals used are well…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: Collecting data that can then be transformed into a bacterial growth curve is usually done using a…

A: A spectrophotometer is an instrument used to measure the amount of light absorbed by a sample. The…

Q: Why can we use drops instead of concentration to graph the data? What do we have to do…

A: concentration:  (1) The quantity of a sub-component (particularly a solute) in a solution. (2) The…

Q: Why are there just colonies and not a lawn of growth on plates (+) LB/AMP/ARA and (+)LB/AMP?

A: The LB+/AMP and LB + AMP/ARA represents the experimental plates having the antibiotic ampicillin in…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: Calculate doubling time (td) and specific growth rate (μ) by using logarithmic growth stage.

A: In unicellular organisms, growth is measured in terms of the increase in the number of cells by cell…

Q: why it is useful to plot the microbial growth from a growing microbial culture?

A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…

Q: Using a dilution series is a way to transfer the very small volumes of original culture necessary to…

A: Answer -To determine the volume of the original bacterial culture required to obtain a specific…

Q: If a bacterial species is not susceptible to an antibacterial drug at the concentration present in a…

A: Antibiotic susceptibility test is the test used to determine the effect of antibiotic to an…

Q: what are some of the strengths and weaknesses to using a Culture dependent enumeration approach from…

A: culture-dependent methods generally recover a small portion of the diversity from soil environments.…

Q: Explain about different types of bioreactors and how they are used in cell suspension culture. What…

A: Ans: Bioreactor It refers to any device that supports the biological environment. This is used for…

Q: The lab provides you with 25 mg/ 100 mL of BAP stock solution. You only need 1 mg of BAP in 180 mL…

A: A substance used for growing and developing microorganisms, cells or tissues in a laboratory setting…

Q: As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the…

A: The number of microorganisms that are able to divide and form new colonies is called a…

Q: Discuss fresh, living preparations vs fixed preparations. What are the advantages and disadvantages…

A: Introduction Microbiology is a branch of biology that deals with the study of microorganisms,…

Q: 3) Define gel electrophoresis, including its theory and application. Describe the steps of running…

A: Introduction:- Electrophoresis is a biophysical technique mainly used to separate proteins and…

Q: How would green manure and soil microorganisms work together in enhancing soil fertility and crop…

A: Green manure crops are advantageous to the soil and future crops. They are grown for the benefits…

Q: What are the advantages/benefit of isometric and allometric growth on an organism?

A: Introduction: Isometric growth:All sections of an organism grow at the same rate, resulting in a…

Q: Place the following in the correct order in terms of steps taken, followed by the consideration…

A: The step and consideration are arranged in a sequence.

Q: How can you tell if the current or the gel electrophoresis is already running and what are the…

A: Gel electrophoresis is the process where DNA, RNA, and protein fragments get separated depending on…

Q: Lysine decarboxylase media is yellow after incubating an organism in it at 37 degrees for 24 hours.…

A: Lysine decarboxylase is an enzyme that is used in decarboxylation test. It is used to distinguish…

Q: You are testing a potential new antibiotic for its effectiveness at killing Streptococcus…

A: Drugs are medications that are taken in the prescribed amounts under the supervision of doctors to…

Q: How to prepare wet mounts and hanging drop mounts of live specimen in microbial motility?

A: Wet mounts and hanging drop mounts of live specimen are used in microbial motility.

Q: temperature and light source affect the number and type of colonies that can be found in the…

A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.