Why do we need to amplify the DNA at all in order to visualize it in a gel? Why run PCR?

Q: In PCR , the primers will determine which gene will amplified (copied) . in lab we’re doing qRT- PCR…

A: One of the most used techniques for quantitatively estimating gene expression is qRT-PCR, often…

Q: Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of…

A: PCR is a scientific method for amplifying a particular DNA segment from just a small portion of…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: You are preparing to amplify a DNA sample using PCR and add the following to your set-up: forward…

A: PCR or polymers chain reaction is a process that use to amplify a particular DNA. It needs different…

Q: When making recombinant DNA, why must you use the same restriction enzyme to cut the gene of…

A: The technique of creating multiple, identical copies of a specific portion of DNA is known as DNA…

Q: Which of the following ingredients does not belong in a PCR reaction? DNA polymerase B) Primer…

A: Polymerase chain reaction is a method used to produce millions of copies of any DNA sample and hence…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: Choose the statements below which are correct. There may be more than one correct statement. A)…

A: PCR or polymerase chain reaction is a process that involves in vitro DNA amplification. It is based…

Q: What's the cloning process from the first step to the last? Put the steps in order based on the…

A: Cloning is a technique for creating a genetically identical copy of a cell or organism. It is a…

Q: What are the disadvantages of doing DNA sequencing?

A: DNA sequencing is a process of identifying the order of nucleic acid bases i.e. adenine, guanine,…

Q: The PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There…

A: PCR is a sensitive technique which helps in making rapid amplification of a specific DNA segment.…

Q: Why is it important to include a selective marker gene on a plasmid? O a) So that you can select…

A: Bacteria are common source of plasmids. For the sole purpose of Cloning experiments, new plasmids…

Q: From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase…

A: Primer is a short piece of ribonucleic acid sequence that provides a starting point for DNA…

Q: E) double stranded DNA separates into single stranded DNA

A: The correct option is E . Double stranded DNA separates into Single stranded DNA .

Q: You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture…

A: While designing an experiment to modify the gene target sequence region is to be known where you…

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: double-stranded length of DNA is exposed to a restriction enzyme.  The enzyme finds 3 recognition…

A: Restriction enzymes Restriction enzymes are the specific enzymes that cleave the DNA in very…

Q: PCR primers are designed to only replicate the N gene sequence of the viral genome. Part of the N…

A: Polymerase chain reaction, or PCR, is a molecular biology technique to make many copies of a…

Q: In PCR, the size of the product is based on A the position of the primers. B) the amount of DNA you…

A: PCR is a method widely used to rapidly make millions to billions of copies of a  specific DNA…