Why do we need to amplify the DNA at all in order to visualize it in a gel? Why run PCR?

Q: what does it mean if our Pcr product was 6.1 nanograms per micrometers and our pcr script was 4.9…

A: PCR amplification is done to clone the desired molecule of DNA or in other words to obtain a large…

Q: In PCR , the primers will determine which gene will amplified (copied) . in lab we’re doing qRT- PCR…

A: One of the most used techniques for quantitatively estimating gene expression is qRT-PCR, often…

Q: Which of the following is necessary for a PCR reaction to proceed? a) the sequence of the ends of…

A: PCR is a scientific method for amplifying a particular DNA segment from just a small portion of…

Q: How does an RT-PCR work?

A: RT- PCR is a technique used in genetic studies used for the detection and quantification of mRNA.…

Q: In rRT–PCR, why do need to convert RNA to DNA first? Why can’t you amplify the RNA molecule…

A: Taq polymerase does not work on RNA samples, so PCR cannot be used to directly amplify RNA…

Q: How do we convert RNA to DNA in RT-PCR?

A: Introduction:- The hereditary ingredient in the cell is genetic material. It contains all of an…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: Which of the following is not denatured by heating steps during PCR?a) DNA polb) RNA polc)…

A: PCR is polymerase chain reaction. It is a technique which is used to make multiple copies of DNA.…

Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…

A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…

Q: You are preparing to amplify a DNA sample using PCR and add the following to your set-up: forward…

A: PCR or polymers chain reaction is a process that use to amplify a particular DNA. It needs different…

Q: Why is PCR beneficial?

A: PCR is a very useful tool and has many applications in various fields such as medical, forensic,…

Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: When making recombinant DNA, why must you use the same restriction enzyme to cut the gene of…

A: The technique of creating multiple, identical copies of a specific portion of DNA is known as DNA…

Q: Which of the following ingredients does not belong in a PCR reaction? DNA polymerase B) Primer…

A: Polymerase chain reaction is a method used to produce millions of copies of any DNA sample and hence…

Q: the following pairings of organelle-function are mistaken loroplast - photosynthesis ell membrane -…

A: It is a sub unit with in a cell that performs a specific function. They are tiny organs and plays a…

Q: Consider the image below. This image shows plasmid DNA isolated through exactly the same method that…

A: Plasmid DNA In contrast to a cell's chromosomal DNA, plasmidsare tiny, circular, double-stranded DNA…

Q: What is PCR bias? Why is it undesirable?

A: Answer: Introduction: Polymerase chain reaction (PCR) is a process extensively used to quickly form…

Q: Can we do PCR on a plasmid?

A: In molecular biology, the plasmid is most abundantly used. The plasmid is a circular autonomously…

Q: Choose the statements below which are correct. There may be more than one correct statement. A)…

A: PCR or polymerase chain reaction is a process that involves in vitro DNA amplification. It is based…

Q: A gene was being ligated to the plasmid vector to prepare a recombinant DNA during bacterial…

A: BASIC INFORMATION  RECOMBINANT DNA TECHNOLOGY  It is formed by attaching the genome of species which…

Q: What's the cloning process from the first step to the last? Put the steps in order based on the…

A: Cloning is a technique for creating a genetically identical copy of a cell or organism. It is a…

Q: What are the disadvantages of doing DNA sequencing?

A: DNA sequencing is a process of identifying the order of nucleic acid bases i.e. adenine, guanine,…

Q: The PCR reaction contains deoxynucleotide triphosphates (dNTPs) in order to construct new DNA. There…

A: PCR is a sensitive technique which helps in making rapid amplification of a specific DNA segment.…

Q: Why is it important to include a selective marker gene on a plasmid? O a) So that you can select…

A: Bacteria are common source of plasmids. For the sole purpose of Cloning experiments, new plasmids…

Q: Can you separate a plasmid from a non plasmid nucleic acid? How?

A: Plasmids are usually the circular, extrachromosomal DNA that has the ability of self replicating…

Q: From where do we get primers for sequencing DNA? A) they are synthesized by reverse transcriptase…

A: Primer is a short piece of ribonucleic acid sequence that provides a starting point for DNA…

Q: What is Recombinant DNA technology ? Who created this ?

A: DNA is the genetic material in all the organisms except certain viruses. It is a polymer of…

Q: E) double stranded DNA separates into single stranded DNA

A: The correct option is E . Double stranded DNA separates into Single stranded DNA .

Q: In your PCR reaction, you were able to use the same primer set, even though you were likely…

A: PCR is a molecular biology tool that is useful in a variety of ways. It is used in forensic…

Q: Why is the use of a heat-stable DNA polymerase important to the success of PCR?

A: The polymerase chain reaction (PCR) is a method used to amplify the deoxyribonucleic acid (DNA) of…

Q: Lane M 1 2 3 4 5 Content DNA ladder Replicate 1 Replicate 2 Replicate 3 Negative control Positive…

A: PCR stands for polymerase chain reaction. PCR is used to amplify nucleic acid segments for further…

Q: You are performing an experiment using CRISPR-cas9 to genetically modify the LacZ gene of a culture…

A: While designing an experiment to modify the gene target sequence region is to be known where you…

Q: How does RT-PCR differ from traditional PCR?

A: Polymerase Chain Reaction (PCR) is a technique that gives us multiple copies of desired DNA…

Q: double-stranded length of DNA is exposed to a restriction enzyme.  The enzyme finds 3 recognition…

A: Restriction enzymes Restriction enzymes are the specific enzymes that cleave the DNA in very…

Q: PCR primers are designed to only replicate the N gene sequence of the viral genome. Part of the N…

A: Polymerase chain reaction, or PCR, is a molecular biology technique to make many copies of a…

Q: In PCR, the size of the product is based on A the position of the primers. B) the amount of DNA you…

A: PCR is a method widely used to rapidly make millions to billions of copies of a  specific DNA…

Q: Why is gel electrophoresis user in DNA fingerprinting?

A: Introduction: It is a test to identify and evaluate genetic information which is the DNA. It is…

Q: Which of the following enzyme is used in PCR? a) EcoRII b) EcoRI c) Tag DNA polymerase d) HRP

A: PCR (Polymerase Chain Reaction) is a widely used technique in molecular biology that amplifies a…