Homework Help is Here – Start Your Trial Now!
Science
Biology
Why are some plates placed into a candle jar?

Why are some plates placed into a candle jar?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question

Q4. Why are some plates placed into a candle jar?

Q6. What is the purpose of each kind of medium?

**Incubation of Plates:** All BHI or blood plates, inside a candle jar, as well as Mitis-salivarius agar plates. The candle jar is lit, closed, and placed in a 37°C incubator. Mannitol salt agar and MacConkey's plates are incubated in the regular 37°C incubator. **Interpretation:** 1. **Remove and Separate Plates:** - All plates are removed from the incubators and separated into two groups—plaque or toothbrush. 2. **Determine Bacteria per Plaque Sample:** - Calculate the number of bacteria per plaque sample. This count will not be per gram as less than a gram of plaque is used. 3. **Determine Bacteria per Toothbrush Head:** - Use counts from blood or BHI to calculate the number of bacteria per toothbrush head. 4. **Group Analysis:** - Calculate the number of bacteria per toothbrush head for each group of bacteria, based on individual media type. 5. **Medium Analysis for Both Toothbrush and Plaque:** - **BAP or BHI:** Total count of facultative bacteria and aerobes. - **Mannitol Salt:** Detects salt-resistant *Staphylococcus* species; *Staph aureus* appears yellow. - **MacConkey's:** Identifies enteric gram-negative rods; coliforms turn red, non-coliforms appear clearish. - **Mitis-salivarius Agar:** - Small blue colonies indicate *Streptococcus mitis*. - Large blue gumdrop-like colonies indicate *Streptococcus salivarius*. - Dark blue/black, shiny colonies indicate *Enterococcus faecalis*. 6. **Gram Stain Procedure:** - Gram stain at least one colony on each type of medium. Record the gram reaction, arrangement, and shape.
Transcribed Image Text:**Incubation of Plates:** All BHI or blood plates, inside a candle jar, as well as Mitis-salivarius agar plates. The candle jar is lit, closed, and placed in a 37°C incubator. Mannitol salt agar and MacConkey's plates are incubated in the regular 37°C incubator. **Interpretation:** 1. **Remove and Separate Plates:** - All plates are removed from the incubators and separated into two groups—plaque or toothbrush. 2. **Determine Bacteria per Plaque Sample:** - Calculate the number of bacteria per plaque sample. This count will not be per gram as less than a gram of plaque is used. 3. **Determine Bacteria per Toothbrush Head:** - Use counts from blood or BHI to calculate the number of bacteria per toothbrush head. 4. **Group Analysis:** - Calculate the number of bacteria per toothbrush head for each group of bacteria, based on individual media type. 5. **Medium Analysis for Both Toothbrush and Plaque:** - **BAP or BHI:** Total count of facultative bacteria and aerobes. - **Mannitol Salt:** Detects salt-resistant *Staphylococcus* species; *Staph aureus* appears yellow. - **MacConkey's:** Identifies enteric gram-negative rods; coliforms turn red, non-coliforms appear clearish. - **Mitis-salivarius Agar:** - Small blue colonies indicate *Streptococcus mitis*. - Large blue gumdrop-like colonies indicate *Streptococcus salivarius*. - Dark blue/black, shiny colonies indicate *Enterococcus faecalis*. 6. **Gram Stain Procedure:** - Gram stain at least one colony on each type of medium. Record the gram reaction, arrangement, and shape.
**Interpretation:** - Determine the total number of bacteria per plaque sample and toothbrush head from BHI. \[ \text{CFU/ mL} = \frac{\text{Number of colonies on a selected plate}}{\text{Total dilution} \times \text{amount plated}} \] **Diagram Explanation:** The image shows results from plates using the plaque sample, arranged in decreasing order of dilution: - **10⁰, 10⁻¹, and 10⁻² Dilutions:** Labelled as TMTC (Too Many To Count), indicating a high bacterial concentration. - **10⁻³ Dilution:** Shows countable colonies. - **10⁻⁴ Dilution:** Contains 238 colonies. - **10⁻⁵ Dilution:** Contains 24 colonies. - **10⁻⁶ Dilution:** Contains 2 colonies. These results help determine the bacterial concentration by calculating Colony Forming Units (CFU) per milliliter.
Transcribed Image Text:**Interpretation:** - Determine the total number of bacteria per plaque sample and toothbrush head from BHI. \[ \text{CFU/ mL} = \frac{\text{Number of colonies on a selected plate}}{\text{Total dilution} \times \text{amount plated}} \] **Diagram Explanation:** The image shows results from plates using the plaque sample, arranged in decreasing order of dilution: - **10⁰, 10⁻¹, and 10⁻² Dilutions:** Labelled as TMTC (Too Many To Count), indicating a high bacterial concentration. - **10⁻³ Dilution:** Shows countable colonies. - **10⁻⁴ Dilution:** Contains 238 colonies. - **10⁻⁵ Dilution:** Contains 24 colonies. - **10⁻⁶ Dilution:** Contains 2 colonies. These results help determine the bacterial concentration by calculating Colony Forming Units (CFU) per milliliter.
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
Nutrient growth medium
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS