Preparing Culture Media (p. 107) o What is media and why are there different kinds? o What are fastidious organisms? o What is Trypticase Soy Broth/Agar? o What is complex media vs. defined media? o How is solid media prepared and what makes it solid?

Q: 7. Use the image to answer the following questions A student collects some of this bacteria to…

A: Answer 1:-  Based on the microbiological examination of the slide shown in the question, the petri…

Q: What are the advantages of staining a bacterial preparation before observingit under a microscope?

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: What's the use of Flagella  mordant for flagella  staining

A: The cells are the primary unit of life. Based on the number of cells an organism may be unicellular…

Q: In lab, one of your classmates is confused because their calculations for bacteria in 1.0 gram of…

A: Here we have to introduce the concept of CFU/mL. This means Colony forming unit. This is the…

Q: Describe two conditions in which bacteria organism will stain Gram variable, that results to Gram…

A: Introduction: The Gram stain is a laboratory technique used to differentiate bacterial species into…

Q: How does plastic effect the growth of bacteria

A: Plastics are the polymers of organic compounds formed from the chains of carbon, sulfur, nitrogen.…

Q: Give two reasons for the differential staining between gram + and gram - organisms

A: Differential Staining is all those staining methods that employ more than one stain to distinguish…

Q: Explain why the light microscope is also called a compound microscope.

A: A microscope is a laboratory device used in the various field of biological sciences for the…

Q: The laboratory that you are working in does not have a microscope or motility medium. Now, how will…

A: The motility of a bacterium is determined by the presence and absence of flagella. The flagellum is…

Q: What is the mostly used parts of the microscope during bacterial experiments and are their roles?

A: Introduction:-  A microscope is an instrument that is used to magnify small objects. Some…

Q: What substances control pigment production in a bacteria cell in microbiology?

A: An organism is a living thing that refers to prokaryotes and eukaryotes. A microscopic organism made…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…

Q: What are bacterial colonies and how are theyformed?

A: Solid media or medium is a liquid or gel which is designed in such a way to provide support for the…

Q: What other properties besides staining are different in grampositive and gram-negative bacteria?

A: Answer- The bacterias can be considered into two different types-  Gram positive bacteria Gram…

Q: What component of bacterial cells helps to combat/regulate osmotic forces?  2. What are Koch’s…

A: Bacteria are one of the most common and versatile organisms on Earth. Their small size, ability to…

Q: What is the color of endospores after Gram staining? After spore staining?

A: There are various staining techniques that are used to stain different cellular structures. The Gram…

Q: how can ultraviolet  be used to control microbial growth. which specific UV can be used to control…

A: Ultraviolet (UV) - non-ionizing radiation. - penetration power is less. - It is a physical mutagen.

Q: What part of the Inverted microscope is different than on a brightfield microscope

A: A microscope is a piece of equipment that magnifies tiny items. Some microscopes can also see the…

Q: When performing a gram stain, what does it tell us about the bacteria and the cell wall speficially?

A: Gram staining is a common approach for distinguishing two broad groups of bacteria based on the…

Q: When observing the endodpore slide in the microscope, what is the difference between endospore and…

A: In spore staining the dye, Malachite green is used for staining the spore, and steaming is used as…

Q: In what ways do the macroscopic features of bacterial colonies differ from those of molds?

A: Molds are a type of fungus. These are small, white, green, orange, purple, or black. They live…

Q: What does the Gram stain reveal about a cell?

A: Gram stain is used for the primary identification of the bacterial cells. In this test, violet dye…

Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…

A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…

Q: A new lab recruit performs their first Gram stain on a slide containing both G(+) and G(-)…

A: Gram staining is a common technique used to differentiate between gram positive and gram negative…

Q: How does staining procedure relates to the benefit endospores provide to cells?

A: Endospore Staining-The main purpose of endospore staining is to differentiate bacterial spores from…

Q: Scientists have discovered a new strains of bacteria but do not know it's morphology. They have also…

A: The bacteria are the unicellular prokaryotic microorganisms and can be studied morphologically by…

Q: How is true microbial motility distinguished from other kinds of motion like Brownian movement that…

A: Q. How is true microbial motility distinguished from other kinds of motion like Brownian movement…

Q: What is the difference between a simple and a compound microscope?

A: A microscope refers to the laboratory instrument that is used to view tiny objects, which cannot be…

Q: What are different ways on how to take care of a microscope?

A: A microscope is a device that magnifies the sample and visualizes the small specimen.

Q: Why does endospore staining require heating? What chemical component of the endospores protects the…

A: Endospore staining is a laboratory technique used to identify endospores, which are highly…

Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…

A: Gram staining helps in differentiation between gram positive and gram negative bacteria. Procedure…

Q: What should be considered when preparing a monolayer cell culture?

A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…

Q: what color are endospores at the conclusion of the endospore stain?

A: Endospores are non-reproductive structures formed by the bacteria when they experience challenging…

Q: Which stains are differential in microbiology?

A: In dis a staining process in which we use more than one chemical stain  to differentiate between…

Q: Explain the roles of lipopolysaccharides in gram negative structure and gram staining.

A: Introduction  Gram staining is a laboratory technique used to differentiate bacterial species into…

Q: Which Two types of electron microscopy are in routine use in microbiology? Explain

A: The electron microscope is an instrument that uses the beam of electrons to examine objects on a…

Q: Explain biologically and chemically how the Gram Stain helps to distinguish between gram-positive…

A: Bacteria are a large group of minute, unicellular, microscopic organisms, which have been classified…

Q: Suppose you are viewing a Gram-stained field of red rods and purple cocci through the microscope.…

A: On the basis of the gram staining technique, the bacteria are classified into Gram-positive and…

Industrial Processes

Industrial processes result in the manufacturing of products (biological products, such as enzymes, medicine) on a large scale using different methods, such as physical and chemical procedures. Few products such as pharmaceuticals, agricultural products, food items, etc., involve microbes and their enzymes. Such processes are termed industrial biological processes. Microbiology and biotechnology enable us to utilize the ability of microorganisms to produce enzymes, primary metabolites, and secondary metabolites, which in turn help to produce the desired products.