Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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
Transcribed Image Text:**Growth Medium Selection Guide:**
This guide helps in selecting the appropriate growth medium for various microbial experiments. Below are the requirements and corresponding media options:
1. **Need to grow a wide range of bacteria and fungi from the environment:**
- **Options:** Choose from available media.
2. **Need to select for the growth of fungi (yeasts & molds):**
- **Options:**
- Sabouraud-Dextrose Agar (SDA)
3. **Need to grow fastidious bacterial pathogens of humans:**
- **Options:**
- SCC Agar (SCCA)
4. **Need to differentiate bacteria based on hemolysis:**
- **Options:**
- Blood Agar (BA)
5. **Need to select for salt tolerant bacteria:**
- **Options:**
- MSA
6. **Need to select for members of genus _Staphylococcus_ in pus from an abscess:**
- **Options:** Choose from available media.
7. **Need to select for members of bacterial family Enterobacteriaceae, e.g., E. coli, Enterobacter, Salmonella, Yersinia, Klebsiella, Proteus:**
- **Options:**
- MacConkey Agar (Mac)
8. **Need to differentiate between lactose fermenting and non-lactose fermenting enterics:**
- **Options:**
- EMB
**Available Media Options:**
- Sabouraud-Dextrose Agar (SDA)
- MacConkey Agar (Mac)
- SCC Agar (SCCA)
- MSA
- Tryptic Soy (TS broth or TSA)
- O'Dougal's Agar (ODA)
- Blood Agar (BA)
- Sacto Agar (SA)
- EMB
This guide assists in optimizing your experimental conditions by choosing the correct growth medium based on the specific microbial requirements.
![## Microbial Differentiation Techniques
### Differentiation between Lactose Fermenting and Non-Lactose Fermenting Enterics
- **Objective**: To identify lactose fermenters and non-fermenters among enteric bacteria.
- **Tool**: [Choose]
### Selection and Differentiation of _E. coli_ from Other Enterobacteriaceae
- **Objective**: To isolate and distinguish _E. coli_ from most other members of the Enterobacteriaceae family.
- **Tool**: [Choose]
### Screening for Fecal Contamination Indicators in Food and Water Samples
- **Objective**: To identify possible fecal contamination in various samples.
- **Tool**: [Choose]
### Distinguishing Mannitol Fermenting and Non-Mannitol Fermenting _Staphylococcus_
- **Objective**: To differentiate between _Staphylococcus_ species based on their ability to ferment mannitol.
- **Tool**: [Choose]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb1d08191-c756-42c9-968c-0042e7e4bfd0%2Fe4532c2a-6b70-46de-9a28-b5aad0b009b9%2Fyhk8qxaw_processed.jpeg&w=3840&q=75)
Transcribed Image Text:## Microbial Differentiation Techniques
### Differentiation between Lactose Fermenting and Non-Lactose Fermenting Enterics
- **Objective**: To identify lactose fermenters and non-fermenters among enteric bacteria.
- **Tool**: [Choose]
### Selection and Differentiation of _E. coli_ from Other Enterobacteriaceae
- **Objective**: To isolate and distinguish _E. coli_ from most other members of the Enterobacteriaceae family.
- **Tool**: [Choose]
### Screening for Fecal Contamination Indicators in Food and Water Samples
- **Objective**: To identify possible fecal contamination in various samples.
- **Tool**: [Choose]
### Distinguishing Mannitol Fermenting and Non-Mannitol Fermenting _Staphylococcus_
- **Objective**: To differentiate between _Staphylococcus_ species based on their ability to ferment mannitol.
- **Tool**: [Choose]
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