Why are some of the detectors listed unsuitable for HPLC? Which of the GC detectors ) in the table below ) are suitable for HPLC?

Q: Why are some of the detectors listed unsuitable for HPLC?

A: HPLC  is applicable for the use with solvent that has UV absorbance. A UV detector cannot be used…

Q: If the TLC plates had been developed in a more polar solvent, would retention factors on the whole…

A:

Q: 23. What would cause compounds with similar structures to be separates on a TLC plate as a…

A: The detail explaination to the above question is given in step 2.

Q: Now that you have experienced both techniques, compare and contrast melting point determination and…

A:

Q: Two methods were applied to measure and test the standard reference materials of chromium. Quantity…

A: For testing that two std deviation are significantly different or not, we apply F test s1 = 0.000013…

Q: What is the Rf value for the uppermost yellow spot in TLC plate 3 (plate imaged below)? It is…

A: The TLC plate 3 with all the spots is given below :

Q: Mass spectrometry and UV/Vis absorbance spectroscopy can both be used as detectors for liquid…

A: Mass spectrometry is an important analytical technique that is used to measure mass to charge ratio.…

Q: Which statement is true? Statement 1: Since tiny amounts of material are exposed on an open…

A: The answer of the above question is given in step 2

Q: Introduction: Column chromatography is an important chro organic compounds. Column chromatography…

A: It is an analytical technique, which is used to seperate a mixture of compound .  In column…

Q: The Ponkan oranges that were imported from one of our neigboring countries are heavily contaminated…

A: working of ELECTRON CAPTURE DETECTOR in GAS CHROMATOGRAPHY

Q: Differentiate between isocratic elution and gradient elution. Why is gradient elution sometimes a…

A:

Q: technique called “GCMS” is often used instead of simple GC analysis. This technique entails passing…

A: GC stands for gas chromatography. It is a chromatography technique used in chemistry. MS stands for…

Q: In general, we want to have absorptions less than or equal to 1.  Given that an ethanol solution of…

A: Given that: absorbance - 1 extinction coefficient (ε) = 23,150 length of light =  1.00 cm  To…

Q: Explain the importance of controlling mobile phase pH in reversed phase HPLC and how this is…

A: When samples contain ionisable compounds, mobile phase pH can be one of the most important variables…

Q: Your lab-mate observes a large amount of streaking and blurring of their samples when they visualize…

A: It is the major problem that YTLC plate seems to be blurred in the UV light.  But you can overcome…

Q: Define evaporative-light scattering detector and its advantages when used with high-precision liquid…

A:

Q: Briefly discuss two types of GC detectors under the following headings a) Theoretical principle b)…

A: Full form of GC detector is Gas Chromatography. GC detectors are the devices which are used in the…

Q: 1. Substances that interact more strongly with the mobile phase will tend to elute out more slowly.…

A: The mobile phase can be either liquid or gas in chromatography. It is passed through a…

Q: D is called____ 2. What is the Rf value of pigment X assuming it travels same distance as your D in…

A: 1.  D is called solvent front  It is the distance travelled by the solvent ( or eluent ) in the TLC…

Q: Chromatography A student in lab used chromatography to separate the pigments present in an extract…

A: Introduction :  We have to calculate retention factor .

Q: - A method for the analysis of a metal ion (M2+) in water from the duck pond at Tech suffers from…

A: Given: Analysis of  metal ion M2+ in water Interfering ion= N+2 Concentration of M2+  = 100 times…

Q: Which statement about TLC analyses is not true? *       A- The line at the base of the TLC plate…

A: TLC is thin layer chromatography.  It is a chromatographic technique to separate the components of…

Q: A sample that contained two components was separated on a column. The column had a length of 15.0…

A: Given: length of column = 15 cm initial resolution = 1.2 required resolution = 2.0 we have to find…

Q: How resolution in HPLC analysis can be improved?

A: HPLC i.e  high-performance liquid chromatography is an analytical method which is being used to…

Q: Which of the following is correct? Select the correct response: HPLC columns are packed with…

A: Hplc

Q: Can you TYPE the definitions of the all of words please Chromatography TLC Stationary phase Mobile…

A: Most of the time, the compounds we encounter are not in their purest form. They are essentially a…

Q: Cuvette 1 2 3 4 Dye Percent Concentration Dye (MM) 100% 75% 50% 25% T 1 Yellow Dye Red Dye…

A: Let us assume the total dye solution after dilution have volume of 100 mL. Initial concentration of…

Q: 3 Compare: a) Capillary Electrophoresis with GC and HPLC. b) Separation Versus Preconcentration.

A:

Q: B. There was big news all over the internet that the Ponkan oranges that were imported from one of…

A: Gas chromatography:       Gas chromatography is an analytical technique used to seperate the…

Q: List the desirable characteristics of HPLC detectors.

A:

Q: During TLC, what is the stationary phase and what is the mobile phase?

A: Chromatography is a laboratory technique for the separation of a mixture into its components. It is…

Q: Compare and contrast GC/MS IR spectrophotometry. TLC spectroscopy, and UV/VIS spectrophotometry Make…

A: Analytical spectroscopy:

Q: correspond

A: HPLC %area values do not corresponds directly to moles because of detector type employed. Generally…

Q: In HPLC, why is purging or initialization being done before analysis of samples, explain.

A: ✓basically following two reasons for using purging and initialization during HPLC sample preparation…

Q: The following calibration data were obtained by an atomic absorption spectrophotometric (AAS) method…

A: To solve this problem, we first need to plot a graph for analytical signals versus concentration.

Q: In column chromatography, what would the effect be of not washing the resin thoroughly enough and…

A: In column chromatography, resin is used for the purification of charged compounds, as well as for…

Q: Explain the importance of controlling mobile phase pH in reversed phase HPLC and how this is…

A: Please find your solution below : Most of the compounds analysed by HPLC contain one or more acidic…

Q: If the selectivity factor is equal to 1, the peaks are separated. True or False?

A: The given statement is If the selectivity factor is equal to 1, the peaks are separated.