Which spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples?     Transmittance for both     Absorbance for both     Absorbance to calibrate and transmittance to read samples     Transmittance to calibrate and absorbance to read samples     Flip a coin to pick a mode each time.

Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?

A: Spectrophotometry is the analytical technique by which the character of a substance is measured…

Q: What are the processes of concentrate samples analysis in Uv-visible spectroscopy?

A: UV-visible spectroscopy is a fast and simple technique that finds the analyte concentration of a…

Q: A radiograph was produced using 80 kVp, 800 mA, .75 seconds and a 72 inches SID. In order to…

A: Given information The radiograph was produced with values: Kilovoltage- 80kVp Quantity of x-rays -…

Q: Why is it important that the standard curve you create in biological analyses with spectrophotometry…

A: The spectrophotometer is an instrument used to measure the amount of light absorbed after it is…

Q: If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints,…

A: Absorbance is the amount of light absorbed by chemical species present in the sample. It is measured…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: he focal length decreases, the angle of light refraction through a convex lens The focal point of…

A: Consider the path of light from its source through specimen into an objective of a microscope. As…

Q: what kind of knfie is used in the following: Rotary Microtome Rocking Microtome Base…

A: Rotary Microtome Profile A or Profile C Diamond Knife (Fixed horizontally)…

Q: Selection of electric current values ​​for electrophoresis. Balance between speed and detail of…

A: Electrophoresis is the process of separation of charged molecules through a gel by applying an…

Q: It is acceptable to use the course focus adjustment on a microscope when the scope is set to high…

A: A part of Microscope: [use]                Under the hood of Coarse & fine adjustment, the…

Q: A radiograph is taken using 400 mA, 10 ms at 90 kV at a 200cm SID. If a new timer setting of 0.04…

A: Radiography is a medical imaging technique that utilizes X-rays to create detailed images of the…

Q: Which of the following method is employed in the sample preparation for the cryo-electron…

A: Cryo-electron microscopy (Cryo-EM) is an advanced imaging technique used to visualize the structures…

Q: The microscopic structure pointed at is . ... the condenser lens the light source • the ocular lens…

A: Microscope is an optical instrument which has major application in visualizing very small to…

Q: Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer…

A: UV-Vis Spectroscopy is also known as Spectrophotometry and is a quantitative technique to determine…

Q: What is reflectance spectrophotometry  What are it's general principles  Reflectance…

A: Answer 1 - Reflectance spectrophotometry is a spectrophotometric technique. Here, from the surface…

Q: Flame Test (Unknown) View the Unknowns to see the results of the flame test. Record Your…

A: As per our honor code, we are not allowed to give you a handwritten solution, however here is the…

Q: Describe the process to get a specimen into view using the 40X objective starting from the point…

A: ExplanationThe first step is to place your slide on the microscope stage. Make sure the specimen on…

Q: f I use the 10x eyepiece and the 40x objective, what is the magnification? Select one: 4 diameters…

A: Eyepiece in a microscope magnifies the primary image produced by objective lens.

Q: Which of the following statements are true about the use of a blank in spectrophotometry? Select all…

A: Spectrophotometers are calibrated by using blank solutions. Basically blank provides the baseline…

Q: For measuring an exact volume of 200 microliters you have to use all options are correct. plastic…

A: Transfer of solutions, medicines, microbial spore suspension etc, require volumes measured to be…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: If a circular object or specimen extends halfway across the scanning field, what isits diameter in…

A: Field of view is defined as the area of the slide that is seen when an observer looks through a…

Q: What are the ways you can control the amount of light passing through your specimen? One way is…

A: A specimen is a sample which represents the whole group. The specimen can anything which is a…

Q: Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a…

A: Resolving power:  An instrument's ability to resolve two points that are near to one another is…

Q: What are 2 advantages to using absorbance values (from a Spectrophotometer) over a spread plate and…

A: Introduction: Spectrophotometry is a method utilized for the evaluation and portrayal of drab mixes…

Q: Provide a list of the names of the devices and technologies used in Dynamic Light Scattering applied…

A: Dynamic Light Scattering (DLS) employs a measurement method to identify the particle size in…

Q: the figure shows the result of the spectral analyzer for paper spectrophotometer without any…

A: Paper spectrophotometry is used in printing industry. In the printing industry, a spectrophotometer…

Q: rovide proper definition and purposes of the ff:  Scanner Low power objective High power objective…

A: Scanner: is a device that optically scans images, and text, and converts them to digital data or…

Q: If the ocular lens of a microscope has 10X magnifying power, and the 40X objective lens is being…

A: Microscope  is an instrument used to visualize minute objects . It  consists of two types of lens…

Q: What is the difference between total magnification and objective magnification? You are looking at…

A: Introduction  A microscope is a scientific instrument that is used to magnify and observe small…

Q: Your spectrophotometer maxes out at an absorbance reading of 2. If you get a reading of 2, what…

A: An analytical tool called a spectrophotometer is used to determine the emission or reflection of…

Q: Question 1 options:    Length of the sample tube   Concentration of the substance   Temperature…

A: The property of the compound to rotate the plane of polarization of the plane polarized light is…

Q: In light microscopy, when magnification is increased, the lens focuses closer to the sample.  This…

A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…

Q: What is the reason for the decrease in the ABS value over time when measuring in the…

A: A spectrophotometer is a device used in an analytical chemistry lab to measures the amount of light…

Q: In the spectrophotometer, why is there a need to measure a 'blank' sample? What will happen to the…

A: A spectrophotometer is one of the most frequent tools used by scientists to determine the presence…

Q: If you have chosen the objective lens with 10x and the ocular lens magnification is also 10x. Then…

A: INTRODUCTION : Objective lens of a compound microscope : A microscope is an instrument which is used…

Q: How does the numerical aperture of an objective lens affect resolution?

A: Numerical aperture of an objective lens denotes the measurement of ability of lens to gather light…

Q: What solution goes into the cuvette when you’re zeroing the spectrophotometer?

A: Spectrophotometer is a device that is used to calculate the absorbance of the light passing through…

Q: Describe how you would prepare the following samples for analysis by a spectroscopic method. You…

A: Spectroscopic method or techniques is the process that recruit the light to link with matter. This…

Q: spectrophotometry, what is the role of the green background in the set-up? What does it represent in…

A: Introduction:- Spectrophotometry is a technique for determining how much light a chemical substance…

Q: Given the following absorbance spectrum, to what wavelength should you set the spectrophotometer to…

A: Spectrophotometer is an instrument that is used to measure the absorption of light (photons) by a…

Q: Which of the following is/are source/s of error in performing a spectrophotometric method? Answer…

A: Spectrophotometery It refers to a technique which is helpful in identifying the solute concentration…

Q: The nurse notes the patient's IV pump is set at 12 mL/hr. The IV bag holds 20 units of Pitocin mixed…

A: Precision and exact calculations are crucial components of patient care for nurses, particularly…

Q: If you place a cuvette with an unknown concentration of that solute in the spectrophotometer and it…

A: The capacity of a substance to absorb light at specific wavelength is called the absorbance. The…