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Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter1: The Clinical Laboratory

Section1.10: The Microscope

Problem 18RQ

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Total magnification of a microscope is determined by
Multiple Choice
dividing the objective magnification value by the ocular value.
multiplying all objective magnification values together.
adding all objective magnification values together.
none of the above.

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Previous question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.
The compound (bright-field) microscope is parfocal. It means that: Question 4 options: objective lenses are built into revolving nosepiece its lenses effectively decrease focal lenght can achieve maximum resolution lower than 0.2 μm image should remain in focus when objective lens is changed
An object found with the 10x objective of the microscope may be viewed with the 40x objective without having to re-focus. This property of the microscope is called: O Confocal O Bifocal Binocular d Parfocal
Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical aperture
briefly describe the functions of the following parts of the microscope: course objective knob, condenser lens, diaphragm, objective lens, illuminator, fine adjustment knob, oil immersion.
The objective lenses of the microscopes used in this course are all parfocal. What does having parfocal objective lenses mean? Question options: The slide has to be moved away from the objective lens (i.e., lowered) when switching between objective lenses. None of these choices The objective lenses have optimized light gathering capability. Once in focus, you may switch between objective lenses with only fine focus adjustment required.
Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…
Complete Table 1 below by supplying the characteristics of each objective. Table 1. Numerical characteristics of microscope objectives. LPO НРО OIO Focal length (mm) Working distance (mm) Linear magnification (X) Numerical aperture (N.A..) Features (color of the band, diameter of the opening of the lens, etc.)
Please explain the table
The following questions talk about increasing magnification in microscopes. Please answer questions 1 and 2 After a specimen is in focus, where in the field of view should position the specimen before increasing the magnification (be precise)? (QUESTION 2 HAS TWO PARTS TO IT): To increase the magnification, which objective should you click into position next? After refocusing the specimen with the new objective, if you want to increase the magnification even more, which objective should you now click into position?
A microscope is turned on, under low power, with a slide positioned on the stage ready to focus. The specimen is visible but not in focus at all. Use the space below to write the correct procedural steps used to find and focus on the specimen under medium power using one of the microscopes in our room.
If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what is the total magnification? 1x 4x 10x 100x 400x

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