Which of the following tools cannot facilitate the visualization of specific intracellular proteins by light or electron microscopy? a.Radioisotopes b.DNA stains c.Enzyme-substrate tracers d.Flourochromes
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Which of the following tools cannot facilitate the visualization of specific intracellular proteins by light or electron microscopy?
d.Flourochromes
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- In sample preparation for electron microscopy, arrange the following steps in correct order A. Apply non-coagulant fixatives to kill cells instantly with minimal structural alterations. B. Wash the specimen with deionized water and then dry it by passing through increasing concentrations of ethanol. C. Expose specimens to uranyl salts to enhance the electron scattering capacity of cellular components. D. Using an ultramicrotome, make ultrathin cuts in the embedded specimen that would be penetrable to electrons. E. Allow the embedding medium to seep into the interstices of the tissue.a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a lot of bacterial colonies in your slide during smear preparation? b. What can you observe in viewing your stained bacterial slide under the microscope if you heat fixed your slide in a much longer exposure to heat?Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information: A. Shape....... gram stain( +) , Gram (-) or both B. Stained color under microscope C. PG Wall thick, thin or both
- Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore StainWhich of the following would be best observed for a bacterial cell using phase-contrast microscopy? a. Motility of cells b. Bacterial nucleoid c. Cell wall d. Glycocalyx
- Which of the following can be used to distinguish one colony from another on an agar plate? Select all that apply. a. Shape b. Color c. Gram stain d. Cell arrangement e. Size f. Elevation g. Margin h. TextureThis is integral to obtaining the experimental data that lead to the development of this model xray crystallography electrodes electron microscope O patch clampDescribe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)
- Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.Examine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..Please discuss why staining is important in visualizing microbes under the microscope. What different types of staining methods can be used?