Which of the following is NOT a direct transfer method for introduction of foreign DNA into the cell? Lütfen birini seçin: O a. Precipitation with Ca O b. Particle bombardment O c. Electroporation O d. Microinjection O e. Viral based method
Q: 6. During penetration of E. coli by a bacteriophage lysozyme is used to allow entry of the…
A: Note:- Sorry, since you have posted multiple questions So we will be solving the first one for you…
Q: in loop streak method ,student inoculate unknown bacteria how many loop this method need ?…
A: The loop streak method is the rapid method of qualitative isolation of discrete colonies. In this…
Q: Which of the following parameters should be altered to increase the amount of vector-insert…
A: In molecular biology research, ligation is a fundamental technique used to join DNA fragments…
Q: Which of the following would most likely be used to lyse bacterial cells so that their nucleic acids…
A: Recombinant DNA technology involves several steps in specific sequence such as isolation of DNA,…
Q: A viral mutation that causes changes in the glycoprotein spikes would affect which aspect of the…
A: A virus consists of three basic structures, an outer protein coating(capsid), an outer protein…
Q: 16. Bacterial cell wall membrane includes all EXCEPT ( Mark both answers ) Group of answer choices…
A: Since you've asked multiple questions, we are only answering the first three questions for you. If…
Q: Which of the following cells has the greatest potential to transfer an entire copy of its chromosome…
A: F+ donor Genes can be transferred from one bacteria to the other by the process of genetic…
Q: Indicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both. 1.…
A: The cyclic reproduction mechanism, by which virulent bacteriophage produces progeny virus by…
Q: The protein of T2 bacteriophage is labeled with 35S and the DNA with 32p. Where would one detect…
A: Introduction: An example of a virus that affects bacteria is the T2 bacteriophage. It belongs to the…
Q: Which of the following would never be an episome? A prophage An F plasmid A provirus A virulent…
A: Episome is a genetic material that can exist independently as well as can be integrated in the main…
Q: 1- which of the following can be used for treating/combating bacterial infections a. targeting…
A: Bacteria are unicellular organisms, having sizes of 0.5 micrometers to 2 micrometers. They cause…
Q: If a bacteria cell is F+, that cell must be capable of transduction. contain the fertility factor.…
A: The microbiology studies about both the diseases causing microbes and beneficiary microbes, about…
Q: Transfer of F factor via bacterial conjugation uses a molecular mechanism related to: a.…
A: Bacterial conjugation may be defined as a process of gene transfer, horizontally, via pilus. The…
Q: Match the terms with their most suitable description. ___ methanogen a. component of…
A: The cell is the basic structural and functional unit of our body. It carries out many functions in…
Q: When phage nucleic acid is incorporated into the nucleic acid of itshost cell and is replicated when…
A: A virus has genetic material that can be deoxyribonucleic acid (DNA) or ribonucleic acid (RNA)…
Q: Proteinaceous toxins known as bacteriocin are produced by the following Col-plasmids. A. Col B,…
A: Introduction Bacteriocins are the proteinaceous or peptidic toxins which are produced by bacteria to…
Q: Conjugation involves which of the following: a. transfer of plasmids among bacteria b. sexual…
A: Conjugation:Conjugation is the process of transferring genetic material from one bacteria to another…
Q: If a viral host cell has a mutation that interferes with the addition of carbohydrates to proteins…
A: It is a multiple choice question.
Q: Mannitol salt agar is a selective media that inhibits the growth of Gram-negative bacteria due to…
A: A Growth medium is an environment created by combining various nutrients that support the growth of…
Q: Which of the following mutant lambda phages can still go through lytic life cycle - (select all…
A: Introduction :- Lambda phage is a bacteriophages that uses Escherichia coli as their host and…
Q: You have two strains of E. coli, Strain I and Strain II. These strains were also co- cultured on a…
A: Antibiotics are those substances that kill or prevent the growth of bacteria. Antibiotics can be of…
Q: A pilus could be used for all of the following except: Motility Attaching to a surface Conjugation…
A: There are several cellular projections found in bacteria that perform several functions. The major…
Q: E. coli Hfr cells Choose an answer below: can transfer genes to F- cells. can form sex pili (F…
A: E.coli Hfr cells are the high frequency cells and during the conjugation process between the Hfr…
Q: a typical eukarytic cell has a diameter of 50 microns while a corona virus particles has a diameter…
A: Given that the diameter of a typical eukaryotic cell = 50 microns the diameter of a coronavirus…
Q: Why are prokaryotes able to replicate so much more quickly than eukaryotes? What cellular components…
A: Prokaryotes are very simple in structure as compared to eukaryotes. Their genome is also very simple…
Q: Halotolerant bacteria grow best in high water activity environments. What protects these bacteria…
A: Halotolerant bacteria: These are archae bacteria which are halophiles (salt loving) and need high…
Q: Which of the following are TRUE when a bacterium engages in conjugation: sex pilus production occurs…
A: Bacterial conjugation is an essential process that allows bacterial cells to exchange genetic…
Q: Which of the three methods of DNA transfer can occur between a bacterium and a plant? O…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: What is the purpose of diluting a culture by making "quadrant streaks" (or "streak plating")…
A: Quadrant streak method is used for isolating the bacteria based on their differences. Four Agar…
Q: Conjugation between bacteria is an example of: O sexual reproduction O asexual reproduction O a…
A: Conjugation between bacteria is an example of: • horizontal transfer
Q: two-day history of burning upon urination. Describes a feeling of urinary urgency and frequency.…
A: The Urinary system works with the help of pair of kidneys. The kidneys consist of numerous nephrons…
Q: What is required for the transformation of a bacterial cell (as opposed to conjugation and…
A: Bacterial transformation is a method of gene transfer by which some bacteria take up foreign genetic…
Q: Part A What kind of inhibition is imposed on HIV protease by ritonavir? O irreversible inhibition O…
A: Enzyme inhibition is when an inhibitor binds to the enzyme at the active site or another site, which…
Q: Pick out the medium (i, ii, iii, or iv) onto whichyou would spread cells from a Lac− Met− E.…
A: Mutants of many different types have been identified in organisms ranging from bacteria to humans.…
Q: Match the definition to its corresponding term. Formation of a pilus to allow genetic exchange…
A: Introduction: The mechanism of gene transfer refers to the movement of genetic information between…
Q: A trp+, arg+ E. coli cell can grow on? A:Minimal medium B:Minimal medium plus amino acids…
A: There are two different kind of bacteria. They are classified as prototroph and auxotroph based on…
Q: Which of the following mushroom-derived molecules is known to block DNA transcription by directly…
A: The mushroom-derived molecule that blocks DNA transcription by directly interfering with the…
Q: A protein you want to isolate is often associated with the cell membrane. After homogenizing your…
A: Ammonium sulphate is often used to either solubilize or precipitate the protein depending on what…
Q: Which of the following does NOT describe a mechanism for drug resistance? Prevention of drug entry…
A: Drug resistance is defined as a reduction in the effectiveness of drugs, chemicals and other agents.…
Q: A bacterial cell receives a new gene contained on a plasmid exchanged through the formation of a sex…
A:
Q: Which of the following would be the most likely consequence for a bacterial colony where the…
A: The question is asking about the consequences of not adding arabinose to the LB plate where bacteria…
Q: During the incubation step of the plaque assay, viral replication leads to host cell lysis. This…
A: The plaque is the clear zone formed by the infection of the virus on the background of the bacterial…
Q: When choosing a "first line" drug to treat an infection, the most important characteristic would be?…
A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…
Q: A bacterial cell must have in order to transfer portions of its chromosome to another cell. O an F…
A: Bacteria are microscopic organisms that come in a variety of shapes and sizes. They can take the…
Q: Which of the following situations leads to lysogeny during phage lambda infection of E. coli?…
A: The λ phage has a double-stranded DNA genome with 12base pairs of single-stranded DNA which are…
Q: The most selectively toxic antibacterial agents are those that interfere with bacterial cell wall…
A: The term "antibacterial" refers to the agents that have the potential to either kill or restrict…
Q: What type of bacterial cell is being depicted in this image 10µm . Gram positive O b. Gram negative
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Which of the following best describes the mechanism of action of the antibiotic streptomycin?…
A: Streptomycin is a broad spectrum of antibiotics that can act against both gram-positive and…
Q: No MIC interpretive standards are given for penicillin G against E. coli. Which ONE of the following…
A: Penicillin G is a beta-lactam antibiotic that is used in the treatment of bacterial infections…
Step by step
Solved in 2 steps
- You perform a Kirby-Bauer assay with two antibiotics. Antibiotic X has a zone of inhibition of 9 mm. Antibiotic Y has a zone of inhibition of 11 mm. Which antibiotic is better at killing this particular microorganism? Group of answer choices 1Antibiotic Y 2Antibiotic X and Y, which have identical antimicrobial activities 3Antibiotic X4 4It is impossible to tell from the information givenBacillis brevis are lysed using a valve-type homogenizer. The extent of disruption depends on the applied pressure and the number of passes through the homogenizer chamber and can be described by the equation, ln(1 − ?) = −????. Is Bacillis brevis a gram-positive or gram-negative bacterial cell? Based on this response, describe the cell wall structure and how it differs from the other type. Develop an equation for calculation of pressure as a function of the number of passes through the homogenizer. What factor(s) did you take into consideration when selecting the number of passes? Escherichia coli cells are lysed with the same homogenization, is this organism gram-positive or gram-negative? What impact does increasing the value of the constant, a, have on the required number of passes at a given pressure? What factors impact the value of a? If fine cell debris after homogenization makes subsequent solid-liquid separation difficult, what would you recommend? Answer as…Choose the one answer that fits best. Which of the following statements regarding bacteria is NOT correct? a. During conjugation, two bacteria exchange genetic information directly via pili b. Bacterial populations have a higher mutation rate because their DNA Polymerases do not have proofreading capability c. Bacterial growth by binary fission is exponential and each division usually takes only a couple of hour or less d. Plasmids carry just a few genes and are replicated independently from the bacterial chromosome e. Cocci that are arranged in clusters/bunches are called staphylococci
- Which form of horizontal gene transfer is shown in the following diagram? Recipient Donor DNA transfer Donor Donor Pilus binding Pilus retraction Stabilization Segregation Transduction O Transformation O Conjugation MacBook Air DDFrom the sequence given below, provide the ff: 5' АтG с с с А сА G TG G A C с тт т с с т GA 3' 1. DNA Complement: 2. RNA strand:A viral mutation that causes changes in the glycoprotein spikes would affect which aspect of the virus? The virus would become lysogenic and incorporate its genome into the host cell genome. The virus would enter the host by a combination of endocytosis and fusion. The virus would lose its viral envelope and become a naked virus. The virus would attach to different host cells and its host range would be altered. The viral genome would not require uncoating once inside the host cell.
- Which of the following is false about counting viable cells? A. CFU(Colony forming unit) are counted instead of individuals cells B. Only 30-300 CFUs are statistically valid C. Colonies growing on the plate represent a fraction of the live cells in the original sample D. You can determine the number of live cells in a broth culture E. It is necessary to multiply the by CFUs to determine the number of viable cells in the original sampleFor a bacterial exotoxin with a typical A-B type toxin structure, removal of the A portion of the toxin would result in which of the following? The B portion remaining would still be able to bind to the target cell, but would not have toxic effects The remaining B portion would enter the cell and carry out its toxic activity, such as ADP-ribosylation The remaining B portion would cause inhibition of protein synthesis Nothing would happen because the A portion is responsible for binding to the target cell. The remaining B portion would still induce cell lysis Please answer asap and in short and content should not be palgarised pleaseanline State- estid%3D1740&strandid%3D&element3D&difficulty=Dassessment&assignment_id%3D42985127&load_test=1&teacherPreview=#test Save Subm gp120 Docking Glycoprotein Lipid Membrane RNA gp41 Transmembrane Glycoprotein Сapsid Matrix Reverse Transcriptase Viruses, although not considered to be alive, attack host cells and cause disease. The attack of a host cell is necessary for the virus to survive for all of the reasons listed EXCEPT one. That is they cannot synthesize proteins becayse they lack ribosomes and must use the ribosomes of their host cells to translate viral messenger RNA into new Eliminate proteins. A) they cannot produce or store energy in the form of ATP and have to get their energy from the host cell. B) they parasitize the host cell for basic molecules like amino acids, nucleotides, and lipids. D) they lack DNA/RNA so they must use the DNA of the host cell. Address 立
- Classify each statement as true of generalized transduction or specialized transduction. Generalized transduction Specialized transduction Answer Bank Genes transfer from random sites in the bacterial chromosome. This utilizes lysogenic phages. Genes that transfer depend on the prophage insertion site. This utilizes lytic phages. Phage inserts itself at a specific site in the bacterial chromosome. This requires accidental packaging of bacterial DNA in viral capsids.The presence of colonies on the bacterial plate post transformation suggests which of the following? Group of answer choices The colonies contain the transformed plasmid and are not resistant to the antibiotic The colonies contain the transformed plasmid and are resistant to the antibiotic The colonies do not contain the transformed plasmid and are not resistant to the antibiotic The colonies do not contain the transformed plasmid and are resistant to the antibioticWhich of the following allows the AIDS virus, which contains RNA to insert viral DNA into the DNA of a host T-cells after the AIDS virus enters the cells? o Receptor proteins located on the surface of the virus o Reverse transcriptase, an enzyme encoded by the virus o The phospholipids found on the envelope of the virus o The protein that makes up the capsid of the virus