In 1966, André Jagendorf and Ernest Uribe directly tested whether artificial proton gradients in isolated chloroplasts could support the generation ofATP without redox energy or light. Chloroplasts were suspended in the dark in low-pH buffer and allowed to equilibrate. When the buffer waschanged to a higher pH and ADP and radiolabeled phosphate (32P) were added, 32p-labeled ATP accumulated in the stroma. Answer the followingquestions on the basis of the results of this experiment:pH 4++Ko comuneOpH1117!!!o↓Measure amount ofsip.ATP synthesizedAllow toequilibrateOC. 8OD. 6Add ADPand pChoose one:ⒸA. 16OB. 12Which of the following is a true representation of the experimental design and results?B.Choose one:A. In order for ATP to be synthesized, the concentration of protons must be higher in the lumen than in thesurrounding chloroplast solution.OB. ATP was used as an energy source to pump protons from the lumen into the stroma.OC. The proton-motive force dissipates in the presence of light.OD. ATP synthase was functioning in reverse.Choose one:AC.How many 32P-ATPs would be produced if 48 H* were transported through the ATP synthase complex in the thylakoid membrane?D.If ADP +P; were completely converted to ATP, and then you continued the experiment to "Step 5° by turning on the lights, what wouldyou predict would be the next picture in the sequence?111Lumen111wwH!!!pH 4www78.8.4Changebufferto pH 8pH 8111the4848pH 4WHAT

Low pH means a higher concentration of H+ ions. The experiment was conducted to determine whether…

Answered: Which of the following is a true…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

In cyclic photophosphorylation, it is estimated that two electrons must be passed through the cycle to pump enough protons to generate one ATP. Assuming that the ΔG for hydrolysis of ATP under conditions existing in the chloroplast is about -50 kJ/mol, what is the corresponding percent efficiency of cyclic photophosphorylation, using light of 700 nm?
DCPIP (2,6-dichlorophenol-indophenol), a blue dye, can act as an electron acceptor and becomes colorless when reduced. A suspension of chloroplasts is prepared, and DCPIP is added to the suspension. The sample is split into two test tubes. One test tube is exposed to light and the other is wrapped in foil (dark control). What would you observe over time comparing the light exposed and the dark control test tubes? A. The sample exposed to light stays blue. The sample kept in the dark turns colorless. B. The sample exposed to light turns colorless. The sample wrapped in foil stays blue. C. Both samples stay blue. D. Both samples turn colorless.
Explain, using a simple diagram, the relationship between acyclic photophosphorylation (photophase) and the Calvin cycle (dark phase).
How do the following features improve the effiency of photosynthesis? A. the presence of accessory pigments b. the original of pigments in a photosystem
Which of the following approaches would be the best way to detect the lack of photosystem II in some photosynthetic organisms? Determine whether they have thylakoid membranes. Determine whether they consume CO2 in the dark. Determine whether they produce O2 in the light. Determine the action spectrum for photosynthesis in these organisms.
Condition: A transmembrane (or integral membrane) protein that acts as a H+ channel has been added to the thylakoid membrane. What impact will thishave to photosynthesis? Explain why. (Specific values are not required, just an indication of overall effect and reasons why.)
A mutant form of an Arabidopsis thaliana plant has defective chlorophyll production, such that the abundance of chlorophyll molecules in chloroplasts is 5% the normal level, and the chlorophyll molecules are on average too far apart for resonance energy transfer to occur. What will be the effect on the overall efficiency of photosynthesis in the plant? Rationalize your answer
Define the following terms:a. glyoxosomeb. coenzyme Ac. NAD(P)+d. FADe. FMN
Calculate the gross rate of photosynthesis (mm/hr) for Elodea if when measuring net photosynthesis, the water level moved up 7mm from the initial level in 10 min, and when measuring cellular respiration it took 15 minutes for the water level to move down 1 mm. 48 mm/hr 42 mm/hr 46 mm/hr 38 mm/hr 50 mm/hr 30 mm/hr
a. What is photosynthesis and what is its importance to plant growth and primary productivity? b. What is the balanced equation for photosynthesis and how does it differ from cellular respiration? c. What are the key components of the light and dark reactions and how do you differentiate one from the other? d. How is photosynthesis measured? e. What are the environmental influences on photosynthesis? f. Include illustrations or figures appropriately. g. List your major references (at least five and no more than 10 references)
UNDER PHOTOSYNTHETIC CONDITIONS, how will the pH of the CO, indicator change? Explain why this happens. What effect will cellular respiration have on the pH? WHY IS THE "dark" cuvette wrapped in foil and then placed under a lamp? HOW DID THE results of the experiment with white light at 25 cm compare to those for blue, green, and red light at the same distance? Is this what you would expect given the composition of white light? UNDER WHICH condition(s) was the CO, indicator the most alkaline? Explain why. WHY DO MANY plants contain accessory pigments other than chlorophyll?
To understand photosynthesis, it is important to first understand the larger picture of what molecules the cell uses and generates and how the different molecules cycle between different reactions. The diagram shows a broad overview of the two stages of photosynthesis. Select the label that best corresponds to the lettered blanks in the diagram below. The parentheses represent the choices for each label on the graph. A. (ADP, ATP, O2, Co2) B. (NADPH, Sugars, NADP+, Sunlight) C. (H2O, Sugars, O2, Co2) D. (Sugars, Co2, O2)

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