In 1966, André Jagendorf and Ernest Uribe directly tested whether artificial proton gradients in isolated chloroplasts could support the generation ofATP without redox energy or light. Chloroplasts were suspended in the dark in low-pH buffer and allowed to equilibrate. When the buffer waschanged to a higher pH and ADP and radiolabeled phosphate (32P) were added, 32p-labeled ATP accumulated in the stroma. Answer the followingquestions on the basis of the results of this experiment:pH 4++Ko comuneOpH1117!!!o↓Measure amount ofsip.ATP synthesizedAllow toequilibrateOC. 8OD. 6Add ADPand pChoose one:ⒸA. 16OB. 12Which of the following is a true representation of the experimental design and results?B.Choose one:A. In order for ATP to be synthesized, the concentration of protons must be higher in the lumen than in thesurrounding chloroplast solution.OB. ATP was used as an energy source to pump protons from the lumen into the stroma.OC. The proton-motive force dissipates in the presence of light.OD. ATP synthase was functioning in reverse.Choose one:AC.How many 32P-ATPs would be produced if 48 H* were transported through the ATP synthase complex in the thylakoid membrane?D.If ADP +P; were completely converted to ATP, and then you continued the experiment to "Step 5° by turning on the lights, what wouldyou predict would be the next picture in the sequence?111Lumen111wwH!!!pH 4www78.8.4Changebufferto pH 8pH 8111the4848pH 4WHAT

Low pH means a higher concentration of H+ ions. The experiment was conducted to determine whether…

Answered: Which of the following is a true…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Transport across the inner membrane of chloroplasts depends on the proton gradient established during ATP synthesis, similar to mitochondria. Can someone explain why this statement is false?
You are a marine biologist sampling an offshore ecosystem. One day, you find a red organism floating in one of your nets. It has structures that resemble the leaves and stems of a plant. You hypothesize that this organism might be photosynthetic. Describe how you could test this hypothesis using a pH indicator solution. a. In detail, describe the steps you would take and the reactants of photosynthesis required. b. What change in pH would indicate that the organism was undergoing photosynthesis, and how would the indicator respond?
Firefly luciferase is the enzyme that allows fireflies to illuminate their abdomens. Because this light generation is an ATP-requiring reaction, firefly luciferase can be used to test for the presence of ATP. In this way, luciferase can test for the presence of life. The coupled reactions are luciferin+O2 ⇌ oxyluciferin+light ATP ⇌ AMP+PPi, ΔGo=−31.6 kJ/mol If the overall ΔGo of the coupled reaction is -8.70 kJ/mol, what is the equilibrium constant, K, of the first reaction at 27oC?
Place numbers beside the statements below to indicate the order of events occurring in the light reactions. I know for sure that 1 and 8 are correct, but the rest I’m not too confident about. I attached both my work and a blank copy.
Complete the following table to compare the electron-transport phase of cellular respiration with the light-dependent reactions of photosynthesis.
If a plant is removed from its natural environment and exposed to 100 percent CO2 composed of the radioisotope 14C, which of the following will not occur? Select one: a. The rate of photorespiration will remain unchanged. b. The sugars synthesized by the plant will be radioactive. c. The oxygen released will also be radioactive. d. All the carbon molecules in the RuBP molecules will eventually become 14C. e. Rubisco will eventually contain 14C.
Choose all that apply: The final electron acceptor(s) associated with photosystem II is/are: NADP+ 02 Photosystem I ATP
Use the data to create a graph in Excel. It should be a scatter plot with a line connecting each point. Looking at the graph: a/ Which wavelengths absorbed most by spinach and likely used for photosynthesis? b/ Which wavelengths are absorbed least?
DCPIP (2,6-dichlorophenol-indophenol), a blue dye, can act as an electron acceptor and becomes colorless when reduced. A suspension of chloroplasts is prepared, and DCPIP is added to the suspension. The sample is split into two test tubes. One test tube is exposed to light and the other is wrapped in foil (dark control). What would you observe over time comparing the light exposed and the dark control test tubes? A. The sample exposed to light stays blue. The sample kept in the dark turns colorless. B. The sample exposed to light turns colorless. The sample wrapped in foil stays blue. C. Both samples stay blue. D. Both samples turn colorless.
Explain, using a simple diagram, the relationship between acyclic photophosphorylation (photophase) and the Calvin cycle (dark phase).
In 150 word count with reference answer the below question The equation for photosynthesis is 6CO2 + 6H2O + Sunlight --> C6H12O6 + 6O2. Explain the role of each of the products (left side of the arrow) and explain where the products (right side of the arrow) came from.
Adjusting the wavelength of light and increasing the temperature can increase the rate of photosynthesis. Using specifics, describe in detail the effect of wavelength and temperature on photosynthesis and why or how these 2 factors affect the creation of the molecules used in or produced by photosynthesis.

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