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When Using I2KI test why should pigments be extracted from the leaf before adding I2KI
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- To observe T. spathacea cells, a sharp blade was used to peel off a thin portion of the lower epidermisof the leaf and was placed on a clean glass slide. One to two drops of 0.01 M NaCl solution was added and a coverslip was placed. The procedure was repeated using 0.30 M NaCl. The leaves were examined under LPO and HPO. A portion with only a single layer of cells was observed. Cells under HPO are shown in your worksheet. Give a short description of the T. spathacea leaf cells in 0.01 M and 0.30 M NaCl solutions. In the figures, label the cell wall, vacuole and tonoplast. Be sure to show the differences between the two leaves and describe them. Notice if the anthocyanin moved out of the vacuole.Staining bacterial capsule can not be accomplished by?Seperation of plant pigment through paper chromatography?
- Using a batch culture method, the effects of 3 types of media on the growth of a plant suspension culture was investigated. Figure below has shown the growth of Murashige and Skoog (MS), Gamborg B5, and Vacin and Went media in plant suspension cultures. Discuss on the findings and draw conclusions. With explanation, recommend which medium is best for maintaining suspension culturesWhy are vegetative cells not stained green after the endospore staining method?Explain the conclussion about gel electrophoresis using 3 different food dyes (yellow, blue and purple).
- Write the procedure for the following 1. Celloidin embedding 2. Vacuum embedding 3. Epoxy embedding 4. resin embeddingExplain the methods and materials in gel electrophoresis using 3 different food dyes (yellow, blue and purple)Descibe shortly four factors which may, as a result of heat generation, have an effect on the effctivity of electrophoretic separation?
- Briefly discuss the difference between simple and differential staining? Which is better suited for common clinical microbiology laboratory applications?Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could’ve caused this? Explain.1a)A surface sterilisation protocol was carried out to sterilise leaf and stemexplants for evaluating the efficacy of the protocol. All explants were washedthoroughly with distilled water before treating with 50% Clorox for 20minutes. The explants were then rinsed with distilled water before the explants were cultured on plates containing Murashige and Skoog (MS) basal medium. A total of five plates were prepared for each type of explant and each plate consisted of 10 explants. The results recorded after a week are shown in Table. Discuss and conclude the results obtained.