What's your next step to characterize this enzyme?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
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6. You are officially in beast-mode. Your thesis deadline is coming up and you are
purifying enzymes like crazy and testing their kinetics. You get the following data for
one of your enzymes.
[S] (µM)
0
1
2.5
5
10
20
50
100
V. (μm/sec)
0
18
46
105
195
450
1218
1841
What's your next step to characterize this enzyme?
Transcribed Image Text:6. You are officially in beast-mode. Your thesis deadline is coming up and you are purifying enzymes like crazy and testing their kinetics. You get the following data for one of your enzymes. [S] (µM) 0 1 2.5 5 10 20 50 100 V. (μm/sec) 0 18 46 105 195 450 1218 1841 What's your next step to characterize this enzyme?
Expert Solution
Introduction

Enzymes are high molecular-weight proteins that catalyse biochemical reactions. They contain an active site where the substrate binds to form a short-lived enzyme-substrate complex that soon dissociates into a product and free enzyme. 

The catalytic activity of an enzyme is a measure of how much product is formed or how much substrate is converted into a product per minute by the enzyme. 

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