What's your next step to characterize this enzyme?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
![6. You are officially in beast-mode. Your thesis deadline is coming up and you are
purifying enzymes like crazy and testing their kinetics. You get the following data for
one of your enzymes.
[S] (µM)
0
1
2.5
5
10
20
50
100
V. (μm/sec)
0
18
46
105
195
450
1218
1841
What's your next step to characterize this enzyme?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F424482ac-ef50-4214-a1e3-aaaeb325792a%2Fc0769efa-44e6-49e8-a1d2-d2d99d756080%2Fhmk7v4_processed.png&w=3840&q=75)
Transcribed Image Text:6. You are officially in beast-mode. Your thesis deadline is coming up and you are
purifying enzymes like crazy and testing their kinetics. You get the following data for
one of your enzymes.
[S] (µM)
0
1
2.5
5
10
20
50
100
V. (μm/sec)
0
18
46
105
195
450
1218
1841
What's your next step to characterize this enzyme?
Expert Solution
Introduction
Enzymes are high molecular-weight proteins that catalyse biochemical reactions. They contain an active site where the substrate binds to form a short-lived enzyme-substrate complex that soon dissociates into a product and free enzyme.
The catalytic activity of an enzyme is a measure of how much product is formed or how much substrate is converted into a product per minute by the enzyme.
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