What technique seperates proteins based on their ionic charge?

Q: A student accidentally added sodium hydroxide (NaOH) to their LB/AMP/ARA total protein sample. Would…

A: Biomolecules that contain both an amino group and a carboxyl group are known as amino acids.…

Q: Give the difference between a proteins tertiary and quaternary structure.

A: Proteins is polypeptide chain which is formed of combination of multiple amino acid joined together…

Q: Select the strongest acid. Succinate (pKa = 5.64) %3D Phenol (pKa = 9.9) Formic acid (pKa = 3.75)…

A: Weak acids are those which dissociate poorly in aqueous solutions while strong acids are those which…

Q: Name and briefly explain two experimental procedures for determining three dimensional structures of…

A: Proteins exhibit hierarchical structure. Primary structure of the protein is the sequence of amino…

Q: What feature of proteins do each of these techniques utilize to identify or separates proteins: gel…

A: It is a laboratory technique for the separation of a mixture by using a solvent on a filter paper.

Q: Why would a protein would be expected to obtain charge from a charged droplet at a later stage of…

A: The electrospray ionization ( ESI ) technique, help to find the molecular mass of the protein, by…

Q: You measure Protein A and Protein B to have a Kd of 10-8 M. At what concentration of Protein B will…

A: Here we have 2 proteins, 'A' and 'B' . The reversible equilibrium between these 2 proteins is shown…

Q: Which type of interaction commonly persists even after urea is added to proteins for denaturation?…

A: Protein is a macromolecule made of amino acids. Several amino acids join together to form a…

Q: Which of the following is CORRECT about protein folding? a)The Levinthal paradox demonstrates that…

A: Protein folding is the process by which the polypeptide chain folds into a characteristic native…

Q: You ran a gel using SDS-PAGE. Total molecular mass of your unknown protein (determined via gel…

A: To determine the molecular weight of subunit C from the SDS-PAGE data, we'll use the provided…

Q: Of the components in the hydrogels beads, which is the protein we are interested in measuring?

A: Hydrogel beads are cross-linked mesh of hydrophilic polymers which is composed of spherical shaped…

Q: Two-dimensional electrophoresis usually exploits which 2 different properties of proteins?

A: Electrophoresis is a technology used in the lab in order to seperate and analyse sample of interest…

Q: What is expected to happen to a suspended protein when the pH of the solution is adjusted to the…

A: The pH impacts the precise nature of protein interactions by altering the protonation state of…

Q: How many amino acids are present in this peptide? فيكم A) 6 B) 7 C) 8 D) 9

A: We have to determine the number of amino acids present in the structure below:

Q: Describe in detail the vapour diffusion method for crystallising a protein

A: Vapor diffusion is the most typically employed method of protein crystallization. In this method,…

Q: COLOR REACTIONS OF PROTEINS AND AMINO ACIDS

A: Amino acids are biomolecules that are comprised of two functional groups, these are an amino group…

Q: A protein is purified from a bacterium using Size Exclusion Chromatography (SEC), with a molecular…

A: It is a sieve tube chromatography technique, in which proteins are separated from other proteins…

Q: Comment on why the feature ~210 nm in Circular Dichroism and Infrared spectroscopy  is useful for…

A: Circular dichroism spectroscopy (CD) is a fundamental insightful method used to investigate…

Q: 1) If the pH of the rain drops from 6 to 5 due to air pollution, the rain has become: a) two time…

A: Acid rain caused when the rain is mixed with the volatile toxic compounds like sulfur dioxide and…

Q: Which tripeptide would you expect to be more water-soluble: INQ or VIW? Fully explain your…

A: Protein monomers are amino acids. An amino acid is a molecule composed of a basic amino group, an…

Q: Both milk and egg whites (albumin) contain proteins. What do your findings suggest for the stability…

A: When an egg is boiled, the heat first breaks (unfolds) the proteins, and then allows the proteins to…

Q: Mass spectrometry and X‑ray diffraction are common biochemical techniques for characterizing…

A: In order to determine the mass-to-charge ratio (m/z) of one or more molecules found in a sample,…

Q: describe the principles behind x-ray diffraction and show how this importnat technique can be used…

A: Proteins are high molecular-weight biomolecules. They are polymers of amino acid residues linked via…

Q: The elution of a protein with an isoelectric point of 7.5, is mostly likely to be affected by a…

A: Chromatography refers to the separation technique that separates substances present in a mixture.…

Q: Please discuss denaturation: Define it in your own words. What type of biological macromolecule is…

A: Some proteins become denatured when food is fried. This is why cooked meat becomes firm and boiled…

Q: 1.0.1 mL of a protein solution of concentration of 11 mg/mL was diluted to a total volume of 4.0 mL…

A: The four types of biological macromolecules are nucleic acids proteins lipids and carbohydrates.…