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What pigments that are present in the spinach leaves correspond to each spot in the tlc chromatography?
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- What is the purpose of adding two drops of acetic acid in thin layer chromatography of plant pigment?In STR typing, the homozygous condition will create a higher peak than the heterozygous condition in terms of relative fluorescence. How do the capillary electrophoresis and the electropherogram correlate?A unique aquatic plant was discovered from a lagoon in El Nido, Palawan. To determine the protein content of the plant, an adequate amount of the plant extract was acquired, and Bradford assay was performed. Determine the total protein concentration, in µg/mL, of the acquired plant extract. A bovine serum albumin (BSA) stock solution with a concentration of 250 µg/mL was used and mixtures with the following compositions and absorbance readings were prepared: Volume of BSA (mL) Volume of water (mL) Absorbance Tube # at 595 nm 1 0.00 2.00 0.000 2 0.20 1.80 0.112 3 0.40 1.60 0.225 4 0.60 1.40 0.318 5 0.80 1.20 0.432 6 1.00 1.00 0.551 The absorbance reading of the plant extract is 0.275.
- What is the form of inflorescence in these figures based on the text?If GUS is present in the Arabidopsis Thaliana plant , what does that mean ? What are the explained results ?When grown in ordinary garden soil and watered normally, a plant engineered to express green fluorescent protein will glow in the dark,whereas a plant engineered to express firefly luciferase will not. Explainthese observations.
- What is the charge of the chromophore/colored ion of the positive staining and negative staining?What two fluorescent proteins would be least appropriate to use to visualize co-localization of two fusion proteins within a cell using fluorescence microscopy? Excitation and Emission Spectra of High-Performance Anthozoa Fluorescent Proteins 100 (a) 100 (b) 5 80 80 60 60 40 40 운 20 20 350 400 450 500 550 600 650 450 500 550 600 650 700 Excitation Wavelength (nm) Emission Wavelength (nm) TagBFP MTFP1 - Azami Green - TagBFP MTFP1 -Azami Green -TagYFP O mKO - mCherry - mPlum - TagYFP - mKO - mCherry mPlum Figure 7 O Tag BFP and mPlum Azami Green and TagYFP O TagYFP and mCherry O All of the combinations would work O TagBFP and mKO Nomalized AbsorptionBoth the isolation buffer and assay buffer that was used in isolation of mitochondria contained 0.3M mannitol. What was the purpose of including mannitol at this concentration?
- Time mapping is performed in a cross involving the genes his, leu, mal, and xyl. The recipient cells were auxotrophic for all four genes. After 25 minutes, mating was interrupted with the following results in recipient cells. Diagram the positions of these genes relative to the origin (O) of the F factor and to one another. (a) 90% were xyl+ (b) 80% were mal+ (c) 20% were his+ (d) none were leu+What are the two main mechanisms by which cells repair doublestrandbreaks? Briefly describe each one.A bloactlve hexapeptide was cleaved by trypsln from a larger proteln. The peptlde was sequenced by treatment with varlous reagents and enzymes. The results are as follows: a) Treatment wlth cyanOgen bromlde gave a tetrapeptlde of compositlon asp, trp, cys and met. b) Treatment with chymotrypsln gave a tripeptlde of compositlon asp, trp and cys, a dipeptlde wlth the same C-term and an amlno acld. c) Treatment with streptococcal protease gave a dipeptlde and a tetrapeptlde. The composltion of the peptlde Is lys, trp, asp, cys and met 1. What Is the sequence of the hexapeptlde? 2. What is the pl of thls peptlde?