What is the amount of sucrose hydrolyzed (in mg/mL) at optimum temperature ?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question

Transcribed Image Text:A sample of yeast extract has been analyzed of its invertase activity. The
effect of temperature on the invertase activity was monitored using the
same dinitrosalicylic acid assay used in your experiment in the laboratory.
Refer to the data below.
What is the amount of sucrose hydrolyzed (in mg/mL) at optimum
temperature ?

Transcribed Image Text:Absorbance
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
-0.1
Temperature (°C)
25
35
45
55
70
90
Absorbance
0.045
0.155
0.210
0.240
0.175
0.075
Sucrose Standard Curve
Amount of sucrose hydrolyzed (mg/mL)
10
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