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please explain this slide
![What is
Recombinant
DNA?
• Recombinant DNA is a DNA molecule composed of one or
more segments from other DNA molecules. The procedure for
doing it is recombinant DNA technology or genetic
engineering.
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- Using the skills you learnt in the DNA Analysis tutorial, correctly identify the gene and the species of the following DNA sequence: AACCAGTGTGCTGCAGGCTGCACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAA Gene: DNA Polymerase II. Species: Mus Musculus Gene: RNA Polymerase I. Species: Homo Sapiens Gene: Insulin Receptor (INSR). Species: Homo Sapiens Gene: Epidermal Growth Factor Receptor (EGFR). Species: Homo SapiensPlease write an introduction to DNA ISOLATION FROM BLOOD containing DNA •DNA structure •DNA isolation •DNA isolation methods •DNA purification •Measurement of DNA concentration •With spectrophotometer •With other methods •Many other topics that you relate with our experimentUsing the skills you learnt in the DNA Analysis tutorial, correctly identify the gene and the species of the following DNA sequence: AACCAGTGTGCTGCAGGCTGCACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAA Using the skills you learnt in the DNA Analysis tutorial, correctly identify the gene and the species of the following DNA sequence: AACCAGTGTGCTGCAGGCTGCACAGGCCCCCGGGAGAGCGACTGCCTGGTCTGCCGCAAA Gene: RNA Polymerase I. Species: Homo Sapiens Gene: Epidermal Growth Factor Receptor (EGFR). Species: Homo Sapiens Gene: DNA Polymerase II. Species: Mus Musculus Gene: Insulin Receptor (INSR). Species: Homo Sapiens
- • Both similar to CS1 • DNAZ Matches Csa LE HE HE 1 CS1 CS2 DNA1 DNA1 DNA2 DNA2 Enz 1 Enzz Enzi Enz2 [1] DI 11 11 TNC (CC which suspects DNA matches that found at the crime scene? Does this automatically mean the suspect is guilty Figure 7.2 Example gel image of suspect and crime scene samples. Samples were digested with restriction enzymes and run on a 0.8% gel. Please note that the markers used match the markersThe following DNA fragment shows where a number of restriction endonucleases cut sites occur within the fragment. The numbers below indicate the distance between those cut sites in base pairs (bp). at of Koni Sall Eo R Pat Bam H Xhot Bem H Pat E Kon 100 200 300 400 s00 s00 700 00 00 1000 1100 1200 1300 1400 1600 1800 1700 1800 100 2000 The gel below shows: • a DNA ladder with markers every 200 bp (DNA Ladder) the resulting restriction fragments of the above DNA fragment if it was completely digested with the restriction endonuclease Xho I (Xho I Digest) the resulting restriction fragments of the above DNA fragment completely digested with unidentified restriction endonuclease(s) (lanes A through F) Which lane shows the DNA fragment completely digested with Pst ? Xho Digest Lanes DNA Ladder A. D F 2000 bp 1800 bp 1600 bp 1400 bp 1200 bp 1000 bp 800 bp 600 bp 400 bp 200 bp ||What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the above
- • Suppose you perform a PCR that begins with one double-strand of the following DNA template: +5'-CTACCTGcoGOTTGACTOCTACCTTcccaGGATaccCAAAArTCTOGAG-3 +3-GATOGACOCCAACTGACGATGGAMGCCCTACOOUTITTAAGGCTC-S'+ A. Draw one cycle of PCR reaction below the following diagram. B. Label the template DNA, the primers, and what is happening at each step. temprature (2) eynteeDNA fragments cut by restriction enzymes can form two types of ends. What are these ends called? • View Available Hint(s) salvage and sharp ends tails and heads sticky and blunt ends ligase and blunt endsIn extracting and Isolating a DNA from yeast and fruit. • What is the role of salt and dishwashing liquid in the extraction/isolation of DNA from yeast and fruit ? EXPLAIN !!
- Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research. Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity. The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c. The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp). Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research. Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity. The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c. The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp). Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…Six step of Recombinant DNA technology
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