What is NOT a feature of PCR? O a. The target can be much larger than the primers. Ob. Primers do not have to be perfectly matched to flanking sequences to amplify targets. O PCR is highly specific because of the stringency of hybridization at relatively high temperature. Od. The sequence of the target needs to be known so that complementary primers can be synthesized. Oe. PCR is exquisitely sensitive; a single DNA molecule can be amplified and detected.
Coding Strand of DNA
When pointing to DNA transcription, the coding strand is found to be the DNA strand whose base sequence is indistinguishable from the base sequence of the RNA transcript developed. It is this strand that comprises the codons, while the non-coding strand comprises the anti-codons.
Nucleotide
Both DNA and RNA are composed of organic molecules known as nucleotides. Hence, nucleotides are known as the basic building blocks of nucleic acids. These substances play a role in various processes such as cell signalling, enzyme reactions, metabolism, and so on.
Structure of Cytosine
Cytosine is among the five primary nitrogenous bases of which DNA and RNA and are being used in storage and transportation of genetic makeup within a cell. Adenine, guanine, thymine as well as uracil are the remaining four nucleobases.
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