What is the role of the substrate? Is it really necessary to add substrate in ELISA?Explain why, or why not. 2. What is the label used in ELISA? Can we use different label? Justify your answer. 3. Explain elaborately the principle involved in ELISA.4. What are the different applications of ELISA? 5. Explain the positive and negative results of ELISA.6. Why do you think it is called indirect ELISA?
1. What is the role of the substrate? Is it really necessary to add substrate in ELISA?Explain why, or why not.
2. What is the label used in ELISA? Can we use different label? Justify your answer.
3. Explain elaborately the principle involved in ELISA.4. What are the different applications of ELISA?
5. Explain the positive and negative results of ELISA.6. Why do you think it is called indirect ELISA?
7. What are the different solid phases that antibody or antigen can bind to,in ELISA?
8. What are the different materials needed in the experiment?
9. What is theimportance of washing? When does washing is performed?
10. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you think the cause of this occurrence? What should the medical technologist do?
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Please answer the ff. questions
7. What are the different solid phases that antibody or antigen can bind to,in ELISA?
8. What are the different materials needed in the experiment?
9. What is theimportance of washing? When does washing is performed?
10. Assuming that these are the 12 microplates. What is wrong with the result of the test? What do you think the cause of this occurrence? What should the medical technologist do?
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