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- 20. Starting with the MacConkey agar and IMVIC tests. Describe how a bacterium can be biochemically identified as Escherichia coli.1. Please discuss how the Methyl Red-Vogues Proskauer test help distinguish microbes from each other.1. Give the importance and purpose of the Cetrimide Test. 2. being performed - Dry Filter Paper Method, Wet Filter Paper Method and Swab Method. 3. In the Oxidative-Fermentative Test, differentiate non-saccharolytic from oxidative from fermentative results.
- Please discuss how the Methyl Red-Vogues Proskauer test helps distinguish microbes from each other.1. Answer the following questions A. What is the antimicrobial mode of action in the antimicrobial assay? B. Based on the results, interpret the efficacy on the test organism?1. Illustrate in a diagram the identification scheme from gram-positive cocci.2. Discuss the principle involved in the biochemical tests in identifying species of staphylococci.3. Illustrate in a diagram the identification scheme for Streptococci.4. Discuss the principle involved in the biochemical tests in identifying species of Streptococci.
- 1. Conclude regarding the chemical composition of the formation of film in fresh milk in biuret test and acrolein test.A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?Discuss what for is the acrolein test and the principle behind the test.
- 1. What are the two ways that nitrate can be reduced by the bacteria. Explain briefly. 2. What organisms are being differentiated in the butyrate disc test. Explain its reaction to the test.From figure shows triplesugar iron agar. What biochemical characteristics does this figureillustrate? How could this medium be used to begin the identificationof the isolated bacteria in this chapter’s Case Study?1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?