UV-Vis Spectroscopy (isopropanol, c=1.60*104 M) 331 nm, A= 1.03 500 nm, A= 0.18 15 Wavelength (nm) Absorbance

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Attatched is UV-Vis Spectrum (Isopropanol, c= 1.65 * 10-4 M)

Include the UV-Vis data in a table with wavelength, absorbance. and molar extinction coefficient 

Wavelength

Absorbance

Molar Extinction Coefficient

300-700 nm

 

 

UV-Vis Spectroscopy (isopropanol, c=1.60*104 M)
JT
JT*
331 nm, A= 1.03
JT*
500 nm, A= 0.18
n
15
Wavelength (nm)
Absorbance
Transcribed Image Text:UV-Vis Spectroscopy (isopropanol, c=1.60*104 M) JT JT* 331 nm, A= 1.03 JT* 500 nm, A= 0.18 n 15 Wavelength (nm) Absorbance
Characterization V
• UV-Vis Spectroscopy
Range: =300-700 nm
Solvent: isopropanol
The compound is weakly polar and dissolving it in isopropanol
is more difficult. So be PATIENT!
• Concentration: based largest peak in the range to be measured:
It is important that the entire sample is dissolve before
any dilution in order to know the true concentration of
the sample being measured in the end!
• Cuvette: polyethylene
• It can only be used with low boiling alcohols.
Toluene, hexane, the solvent mixture used for recrystallization, acetone,
etc. cannot be used with this cuvette because they will
etch the cuvette making it non-transparent.
14
Transcribed Image Text:Characterization V • UV-Vis Spectroscopy Range: =300-700 nm Solvent: isopropanol The compound is weakly polar and dissolving it in isopropanol is more difficult. So be PATIENT! • Concentration: based largest peak in the range to be measured: It is important that the entire sample is dissolve before any dilution in order to know the true concentration of the sample being measured in the end! • Cuvette: polyethylene • It can only be used with low boiling alcohols. Toluene, hexane, the solvent mixture used for recrystallization, acetone, etc. cannot be used with this cuvette because they will etch the cuvette making it non-transparent. 14
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