Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample. Number of CFUS = CFUS You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original undiluted sample. Lab Data CFUS Number of CFUS Phase 1
Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample. Number of CFUS = CFUS You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original undiluted sample. Lab Data CFUS Number of CFUS Phase 1
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Need help figuring out the top two CFU questions. Unsure of how to calculate

Transcribed Image Text:Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample.
CFUS
Number of CFUS =
You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired,
re-calculate the number of CFUs in the original undiluted sample.
Lab Data
Number of CFUs =
CFUS
Why is it necessary to dilute a sample in order to determine bacteri
O A. Dilution ensures that there are enough nutrients in the medium for gro
O B. The dilution process encourages the growth of more and different type
C. The bacterial density in the original sample may be too high for the for
O D. The dilution process is conducted to save time when counting.
While moonlighting in the hospital lab, you receive an order for a ra
based on a quantitative urine culture yielding greater than 100,000
come back from incubation the next day, but many of your plates w
you to count. It yields 102 isolated colonies from a 0.1 mL inoculur
METHODS
Phase 1
Plate
#
1
2
3
4
Image
Dilution Label
1:10,000
1:100,000
1:1,000,000
1:10,000,000
Phase 2
Growth Appearance on Plate
Heavy
Heavy
Intermediate
Scant
Plate 1
Sample Dilution
Colony Count
You Can
Plate Selected For Counting
Volume of Diluted Sample (mL)
DA How to Cala
Plate 2
No
Isolated Colonies
Present?
Yes, few
Yes, many
Yes, few
000,cogli
Plate 3
Countable?
Too numerous to count
Too numerous to count
Countable
Too few to count
000,000,01:1
Plate 4
Plate 3
1
1:1,000,000
46
- X
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