Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample. Number of CFUS = CFUS You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original undiluted sample. Lab Data CFUS Number of CFUS Phase 1

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Need help figuring out the top two CFU questions. Unsure of how to calculate 

Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample.
CFUS
Number of CFUS =
You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired,
re-calculate the number of CFUs in the original undiluted sample.
Lab Data
Number of CFUs =
CFUS
Why is it necessary to dilute a sample in order to determine bacteri
O A. Dilution ensures that there are enough nutrients in the medium for gro
O B. The dilution process encourages the growth of more and different type
C. The bacterial density in the original sample may be too high for the for
O D. The dilution process is conducted to save time when counting.
While moonlighting in the hospital lab, you receive an order for a ra
based on a quantitative urine culture yielding greater than 100,000
come back from incubation the next day, but many of your plates w
you to count. It yields 102 isolated colonies from a 0.1 mL inoculur
METHODS
Phase 1
Plate
#
1
2
3
4
Image
Dilution Label
1:10,000
1:100,000
1:1,000,000
1:10,000,000
Phase 2
Growth Appearance on Plate
Heavy
Heavy
Intermediate
Scant
Plate 1
Sample Dilution
Colony Count
You Can
Plate Selected For Counting
Volume of Diluted Sample (mL)
DA How to Cala
Plate 2
No
Isolated Colonies
Present?
Yes, few
Yes, many
Yes, few
000,cogli
Plate 3
Countable?
Too numerous to count
Too numerous to count
Countable
Too few to count
000,000,01:1
Plate 4
Plate 3
1
1:1,000,000
46
- X
Transcribed Image Text:Using the count data and observational data you acquired, calculate the number of CFUs in the original undiluted sample. CFUS Number of CFUS = You discover that the plate you selected had only been inoculated with 0.1mL of the dilution instead of 1mL. Using the count data and observational data you acquired, re-calculate the number of CFUs in the original undiluted sample. Lab Data Number of CFUs = CFUS Why is it necessary to dilute a sample in order to determine bacteri O A. Dilution ensures that there are enough nutrients in the medium for gro O B. The dilution process encourages the growth of more and different type C. The bacterial density in the original sample may be too high for the for O D. The dilution process is conducted to save time when counting. While moonlighting in the hospital lab, you receive an order for a ra based on a quantitative urine culture yielding greater than 100,000 come back from incubation the next day, but many of your plates w you to count. It yields 102 isolated colonies from a 0.1 mL inoculur METHODS Phase 1 Plate # 1 2 3 4 Image Dilution Label 1:10,000 1:100,000 1:1,000,000 1:10,000,000 Phase 2 Growth Appearance on Plate Heavy Heavy Intermediate Scant Plate 1 Sample Dilution Colony Count You Can Plate Selected For Counting Volume of Diluted Sample (mL) DA How to Cala Plate 2 No Isolated Colonies Present? Yes, few Yes, many Yes, few 000,cogli Plate 3 Countable? Too numerous to count Too numerous to count Countable Too few to count 000,000,01:1 Plate 4 Plate 3 1 1:1,000,000 46 - X
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