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Use the gel image below to determine the sequence of the original DNA template strand sequenced using Sanger sequencing from the 5' end to the 3' end.

Use the gel image below to determine the sequence of the original DNA template strand sequenced using Sanger sequencing from the 5' end to the 3' end.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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**Using Gel Electrophoresis to Determine DNA Sequence** This image shows a gel electrophoresis result used to determine the sequence of an original DNA template strand via Sanger sequencing. The gel has four lanes labeled A, C, G, and T, corresponding to the different dideoxynucleotides that terminate DNA synthesis at adenine, cytosine, guanine, and thymine, respectively. **Description of the Gel:** - **Lane A**: Contains bands indicating DNA fragments that end in adenine. - **Lane C**: Contains bands indicating DNA fragments that end in cytosine. - **Lane G**: Contains bands indicating DNA fragments that end in guanine. - **Lane T**: Contains bands indicating DNA fragments that end in thymine. The largest fragments are at the top of the gel (-), and the smallest are at the bottom (+), indicating the 5' to 3' direction. **Gel Analysis for DNA Sequence:** By reading the bands from the bottom to the top, the DNA sequence is determined as "TAC GGT CAT" from the 5' end to the 3' end. **Choices provided:** - TAC GGT CAT (highlighted choice) - TAC TGG CAT - ATG CCA GTA - ATG ACC GTA - TAC GGT TAC The correct sequence, confirmed by the gel, is "TAC GGT CAT."
Transcribed Image Text:**Using Gel Electrophoresis to Determine DNA Sequence** This image shows a gel electrophoresis result used to determine the sequence of an original DNA template strand via Sanger sequencing. The gel has four lanes labeled A, C, G, and T, corresponding to the different dideoxynucleotides that terminate DNA synthesis at adenine, cytosine, guanine, and thymine, respectively. **Description of the Gel:** - **Lane A**: Contains bands indicating DNA fragments that end in adenine. - **Lane C**: Contains bands indicating DNA fragments that end in cytosine. - **Lane G**: Contains bands indicating DNA fragments that end in guanine. - **Lane T**: Contains bands indicating DNA fragments that end in thymine. The largest fragments are at the top of the gel (-), and the smallest are at the bottom (+), indicating the 5' to 3' direction. **Gel Analysis for DNA Sequence:** By reading the bands from the bottom to the top, the DNA sequence is determined as "TAC GGT CAT" from the 5' end to the 3' end. **Choices provided:** - TAC GGT CAT (highlighted choice) - TAC TGG CAT - ATG CCA GTA - ATG ACC GTA - TAC GGT TAC The correct sequence, confirmed by the gel, is "TAC GGT CAT."
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