Unpacking Problem 411. What type of organism is E. coli?2. What does a culture of E. coli look like?3. On what sort of substrates does E. coli generally grow inits natural habitat?4. What are the minimal requirements for E. coli cells todivide?5. Define the terms prototroph and auxotroph.6. Which cultures in this experiment are prototrophic,and which are auxotrophic?7. Given some strains of unknown genotype regardingthiamine and proline, how would you test their genotypes? Give precise experimental details, includingequipment.8. What kinds of chemicals are proline and thiamine?Does it matter in this experiment?9. Draw a diagram showing the full set of manipulationsperformed in the experiment.10. Why do you think the experiment was done?11. How was it established that pro enters after thi? Giveprecise experimental steps.12. In what way does an interrupted-mating experimentdiffer from the experiment described in this problem?13. What is an exconjugant? How do you think that exconjugants were obtained? (It might include genes not described in this problem.)14. When the pro gene is said to enter after thi, does it meanthe pro allele, the pro+ allele, either, or both?15. What is “fully supplemented medium” in the context ofthis question?16. Some exconjugants did not grow on minimal medium.On what medium would they grow?17. State the types of crossovers that take part in Hfr × Frecombination. How do these crossovers differ fromcrossovers in eukaryotes?18. What is a recombination unit in the context of the present analysis? How does it differ from the map unitsused in eukaryote genetics?
Unpacking Problem 41
1. What type of organism is E. coli?
2. What does a culture of E. coli look like?
3. On what sort of substrates does E. coli generally grow in
its natural habitat?
4. What are the minimal requirements for E. coli cells to
divide?
5. Define the terms prototroph and auxotroph.
6. Which cultures in this experiment are prototrophic,
and which are auxotrophic?
7. Given some strains of unknown genotype regarding
thiamine and proline, how would you test their genotypes? Give precise experimental details, including
equipment.
8. What kinds of chemicals are proline and thiamine?
Does it matter in this experiment?
9. Draw a diagram showing the full set of manipulations
performed in the experiment.
10. Why do you think the experiment was done?
11. How was it established that pro enters after thi? Give
precise experimental steps.
12. In what way does an interrupted-mating experiment
differ from the experiment described in this problem?
13. What is an exconjugant? How do you think that exconjugants were obtained? (It might include genes not described in this problem.)
14. When the pro gene is said to enter after thi, does it mean
the pro allele, the pro+ allele, either, or both?
15. What is “fully supplemented medium” in the context of
this question?
16. Some exconjugants did not grow on minimal medium.
On what medium would they grow?
17. State the types of crossovers that take part in Hfr × Frecombination. How do these crossovers differ from
crossovers in eukaryotes?
18. What is a recombination unit in the context of the present analysis? How does it differ from the map units
used in eukaryote genetics?
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