Under anoxic conditions, biological denitrification of waste water by activated sludge results in theconversion of nitrate to nitrogen gas. Sludge is microorganisms, and these microorganisms carry outthis reaction, this is a biological reaction. When acetic acid provides the carbon source, the reactioncan be represented as follows:5CH3COOH + 8NO3 → 4N2+ 10CO2 + 6H20 + 8HOAcetic acidNitratea) Is the stoichiometric equation balanced? write down the number of atoms of each species on leftside and also on the right side of this equation.b) In the absence of side reactions, what is the yield of nitrogen from acetic acid in g g1?c) A certain waste water contains 6.0 mM acetic acid and 7mM NaNO3. If 25% of the acetic acid and15% of the nitrate (NO3) are consumed in other reactions (e.g, for growth of the microorganisms inthe sludge), which is the limiting substrate in the denitrification reaction?d) For the situation described in part c), what mass of gaseous nitrogen (N2) in grams is producedfrom treatment of 5000 liters of waste water if the reaction is allowed to proceed until the limitingsubstrate is exhausted? What is the extent of reaction § under these conditions?

(a) The equation is stochimetrically balanced. The number of atoms of an element on left side is…

Answered: Under anoxic conditions, biological…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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L-lysine is being produced by Corynebacterium glutamicum in a bioreactor using glucose as the carbon source, ammonia as the nitrogen source. The bacteria grow aerobically in a continuous process at steady-state. All streams of this process are aqueous. In the inlet stream, 8 grams of glucose is included per 100 grams of stream liquid, which flows at a rate of 500 kg/h. The flow rate of glucose in the outlet steam is 1 kg/h. The reaction occurs at 25 °C.What is the mole percentage of glucose that was consumed during cell growth inside the bioreactor?Does the growth of Corynebacterium glutamicum cells inside this bioreactor release or absorbheat? Should heat be supplied to the bioreactor, or removed from, to maintain the operation temperature?
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The following four chemicals commonly found in defined growth media contain all of the necessary atomic macronutrients, namely C, O, N, H, S and P, which microbes require: glucose (C6H12O6), K2HPO4, MgSO4.7H2O, and (NH4)HPO4. Part a. Create a simple diagram to map which growth media chemical contributes atoms to which of the four biological macromolecules (amino acids, polysaccharides, lipids, and ribonucleic acids). Part b. Consider a cell culture broth that originally (before inoculation with cells) contains 5.0 gr/L glucose (C6H12O6), 1.2 gr/L K2HPO4, 0.3 gr/L MgSO4∙7H2O, and 1.0 gr/L (NH4)HPO4. Create a table showing how much of each of the different atoms (C, H, O, K, P, Mg, S, N) are available for incorporation into cell macromolecules (assume 50% of the glucose is catabolized, while the remainder goes for energy (respiration). Express your answers with units of grams of atomic species / liter of culture broth.
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