Under anoxic conditions, biological denitrification of waste water by activated sludge results in the conversion of nitrate to nitrogen gas. Sludge is microorganisms, and these microorganisms carry out this reaction, this is a biological reaction. When acetic acid provides the carbon source, the reaction can be represented as follows: 5CH3COOH + 8NO3 → 4N2+ 10CO2 + 6H20 + 8HO Acetic acid Nitrate a) Is the stoichiometric equation balanced? write down the number of atoms of each species on left side and also on the right side of this equation. b) In the absence of side reactions, what is the yield of nitrogen from acetic acid in g g1? c) A certain waste water contains 6.0 mM acetic acid and 7mM NANO3. If 25% of the acetic acid and 15% of the nitrate (NO3) are consumed in other reactions (e.g, for growth of the microorganisms in the sludge), which is the limiting substrate in the denitrification reaction? d) For the situation described in part c), what mass of gaseous nitrogen (N2) in grams is produced from treatment of 5000 liters of waste water if the reaction is allowed to proceed until the limiting substrate is exhausted? What is the extent of reaction § under these conditions?

Biochemistry
9th Edition
ISBN:9781319114671
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Chapter1: Biochemistry: An Evolving Science
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Under anoxic conditions, biological denitrification of waste water by activated sludge results in the
conversion of nitrate to nitrogen gas. Sludge is microorganisms, and these microorganisms carry out
this reaction, this is a biological reaction. When acetic acid provides the carbon source, the reaction
can be represented as follows:
5CH3COOH + 8NO3 → 4N2+ 10CO2 + 6H20 + 8HO
Acetic acid
Nitrate
a) Is the stoichiometric equation balanced? write down the number of atoms of each species on left
side and also on the right side of this equation.
b) In the absence of side reactions, what is the yield of nitrogen from acetic acid in g g1?
c) A certain waste water contains 6.0 mM acetic acid and 7mM NaNO3. If 25% of the acetic acid and
15% of the nitrate (NO3) are consumed in other reactions (e.g, for growth of the microorganisms in
the sludge), which is the limiting substrate in the denitrification reaction?
d) For the situation described in part c), what mass of gaseous nitrogen (N2) in grams is produced
from treatment of 5000 liters of waste water if the reaction is allowed to proceed until the limiting
substrate is exhausted? What is the extent of reaction § under these conditions?
Transcribed Image Text:Under anoxic conditions, biological denitrification of waste water by activated sludge results in the conversion of nitrate to nitrogen gas. Sludge is microorganisms, and these microorganisms carry out this reaction, this is a biological reaction. When acetic acid provides the carbon source, the reaction can be represented as follows: 5CH3COOH + 8NO3 → 4N2+ 10CO2 + 6H20 + 8HO Acetic acid Nitrate a) Is the stoichiometric equation balanced? write down the number of atoms of each species on left side and also on the right side of this equation. b) In the absence of side reactions, what is the yield of nitrogen from acetic acid in g g1? c) A certain waste water contains 6.0 mM acetic acid and 7mM NaNO3. If 25% of the acetic acid and 15% of the nitrate (NO3) are consumed in other reactions (e.g, for growth of the microorganisms in the sludge), which is the limiting substrate in the denitrification reaction? d) For the situation described in part c), what mass of gaseous nitrogen (N2) in grams is produced from treatment of 5000 liters of waste water if the reaction is allowed to proceed until the limiting substrate is exhausted? What is the extent of reaction § under these conditions?
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