Tris can be purchased in two forms, the acid form (Tris HCl, 157.6 g/mol) and the base form (Tris base, 121.14 g/mol). You can make an appropriate buffer by mixing the correct ratio of acid and base, as stated above.  Calculate how many moles of TOTAL Tris (acid + base) you will need to make 40 mL of buffer. Calculate the number of moles of Tris acid and the number of moles of Tris base you will need in the buffer using the Henderson-Hasselbalch equation. Calculate the mass of Tris acid and Tris base needed.

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Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Tris can be purchased in two forms, the acid form (Tris HCl, 157.6 g/mol) and the base form (Tris base, 121.14 g/mol). You can make an appropriate buffer by mixing the correct ratio of acid and base, as stated above. 

  1. Calculate how many moles of TOTAL Tris (acid + base) you will need to make 40 mL of buffer.

  2. Calculate the number of moles of Tris acid and the number of moles of Tris base you will need in

    the buffer using the Henderson-Hasselbalch equation.

  3. Calculate the mass of Tris acid and Tris base needed.

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