Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation(Salting Out)After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4.Why is there a need to gradually add the ammonium sulfate to the sample? Table 2.1.iSODUOImaes™table (grams per 1L sample).104070 80 905060100Grams of solid (NH4)2SO4 to be added to 1 L of solution176(242 314 390 472 561 657551376157117182251 325 405 491 5846852059188 260121337 4215116093061126195 270 351 4385334063130202281365457(NH 504)2s 6065135 210 | 29238167140 2193057070146228satu 80731529076

The experiment is about isolation of crude ovalbumin, which is a protein.

Answered: Topic: Isolation of Crude Ovalbumin…

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

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Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation
(Salting Out)

After filtration, 30.0 mL of the filtrate was obtained, and the solution was brought from 0% to 40% saturation by adding the required amount of powdered ammonium sulfate, (NH4)2SO4.

Why is there a need to gradually add the ammonium sulfate to the sample?

Table 2.1.
iSODUO
Imaes™
table (grams per 1L sample).
10
40
70 80 90
50
60
100
Grams of solid (NH4)2SO4 to be added to 1 L of solution
176(242 314 390 472 561 657
55
13
761
57
117
182
251 325 405 491 584
685
20
59
188 260
121
337 421
511
609
30
61
126
195 270 351 438
533
40
63
130
202
281
365
457
(NH 50
4)2s 60
65
135 210 | 292
381
67
140 219
305
70
70
146
228
satu 80
73
152
90
76

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