The sequence of a 29 aa long peptide can be determined from the following data. a) Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val. b) Trypsin digestion, separation of peptides and Edman degradation give sequences for peptide fragments as follows: Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu Ala-Ala-Trp-Gly-Lys Val-Leu-Ser-Pro-Ala-Lys Thr-Asn-Val-Lys

Biochemistry
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ISBN:9781319114671
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Chapter1: Biochemistry: An Evolving Science
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The
sequence
of a 29 aa long peptide can be determined from the following data.
a) Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val.
b) Trypsin digestion, separation of peptides and Edman degradation give sequences for peptide
fragments as follows:
Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu
Ala-Ala-Trp-Gly-Lys
Val-Leu-Ser-Pro-Ala-Lys
Thr-Asn-Val-Lys
c) Chymotrypsin digestion, separation of peptides, and Edman degradation give the following
sequences for fragments:
Gly-Ala-Glu-Ala-Thr-Glu
Gly-Lys-Val-Gly-Ala-His-Ala-Gly-Glu-Tyr
Val-Leu-Ser-Pro-Ala-Lys-Thr-Asn-Val-Lys-Ala-Ala-Trp
What is the sequence for the peptide?
Transcribed Image Text:The sequence of a 29 aa long peptide can be determined from the following data. a) Treatment of the peptide with dansyl chloride reveals that the amino terminal is Val. b) Trypsin digestion, separation of peptides and Edman degradation give sequences for peptide fragments as follows: Val-Gly-Ala-His-Ala-Gly-Glu-Tyr-Gly-Ala-Glu-Ala-Thr-Glu Ala-Ala-Trp-Gly-Lys Val-Leu-Ser-Pro-Ala-Lys Thr-Asn-Val-Lys c) Chymotrypsin digestion, separation of peptides, and Edman degradation give the following sequences for fragments: Gly-Ala-Glu-Ala-Thr-Glu Gly-Lys-Val-Gly-Ala-His-Ala-Gly-Glu-Tyr Val-Leu-Ser-Pro-Ala-Lys-Thr-Asn-Val-Lys-Ala-Ala-Trp What is the sequence for the peptide?
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