The replication of DNA begins at a special site with the formation of leading to the molecule forming into two . . for the synthesis .... and the other is single strands. These strands then act as 2. of new DNA. One strand is called the 3.. called the ... During DNA replication 5.. bases along the two strands. align with complementary

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Scientists investigated DNA replication by repeating the experiments of Meselson
and Stahl. They grew bacteria for several generations in a nutrient solution
containing a heavy form of nitrogen (N). The bacteria integrated the heavy
nitrogen into their DNA as part of the DNA replication process. DNA was then
isolated from a sample of these bacterial cells.
The scientists then transferred some of the bacteria (grown in 15N) to a nutrient
solution containing the lighter form of nitrogen (14N). The bacteria were allowed
to grow and divide. After growth of one cell division in the 1"N medium, DNA was
isolated from a sample of these bacteria. A further sample of DNA was then
obtained from cells grown for two further divisions in the 14N medium.
The DNA samples from the bacteria grown in 15N and 14N were transferred to
separate tubes, and suspended in a solution of sucrose. These tubes were
centrifuged (spun) at the same speed and for the same length of time, and
different bands of DNA settled at different places in each tube.
Figure 1 below shows an example of the scientists' results.
Figure 1
Band containing
DNA from the
sample of
bacteria
Solution
Sample 1
Bacteria grown for
several generations
in a nutrient soltion
containing 1N
Sampie 2
Bacteria grown ina
nuient solution
containing "N for
one oell division
Sample 3
Bacteria grown in a
nutrient solution
containing N for
wo cell divisions
Table 1 below shows the types of DNA molecule (A, B and C) that could
be present in samples 1, 2 and 3 above.
a)
Table 1
Type(s) of DNA molecule
present in each sample
15N 15N
15N 14N
14N 14N
Sample
A
B
C
1
2
Transcribed Image Text:Scientists investigated DNA replication by repeating the experiments of Meselson and Stahl. They grew bacteria for several generations in a nutrient solution containing a heavy form of nitrogen (N). The bacteria integrated the heavy nitrogen into their DNA as part of the DNA replication process. DNA was then isolated from a sample of these bacterial cells. The scientists then transferred some of the bacteria (grown in 15N) to a nutrient solution containing the lighter form of nitrogen (14N). The bacteria were allowed to grow and divide. After growth of one cell division in the 1"N medium, DNA was isolated from a sample of these bacteria. A further sample of DNA was then obtained from cells grown for two further divisions in the 14N medium. The DNA samples from the bacteria grown in 15N and 14N were transferred to separate tubes, and suspended in a solution of sucrose. These tubes were centrifuged (spun) at the same speed and for the same length of time, and different bands of DNA settled at different places in each tube. Figure 1 below shows an example of the scientists' results. Figure 1 Band containing DNA from the sample of bacteria Solution Sample 1 Bacteria grown for several generations in a nutrient soltion containing 1N Sampie 2 Bacteria grown ina nuient solution containing "N for one oell division Sample 3 Bacteria grown in a nutrient solution containing N for wo cell divisions Table 1 below shows the types of DNA molecule (A, B and C) that could be present in samples 1, 2 and 3 above. a) Table 1 Type(s) of DNA molecule present in each sample 15N 15N 15N 14N 14N 14N Sample A B C 1 2
Using your knowledge of the Meselson and Stahl experiment and semi-
conservative replication of DNA show which DNA is found in samples 1, 2
and 3.
In your answer booklet, please write the missing words from the
statement below. The missing words should be clearly labelled with the
relevant number.
The replication of DNA begins at a special site with the formation of
a'.
single strands. These strands then act as 2 . for the synthesis
of new DNA. One strand is called the 3.
called the 4..
During DNA replication 5.
bases along the two strands.
leading to the molecule forming into two
.... and the other is
.align with complementary
Transcribed Image Text:Using your knowledge of the Meselson and Stahl experiment and semi- conservative replication of DNA show which DNA is found in samples 1, 2 and 3. In your answer booklet, please write the missing words from the statement below. The missing words should be clearly labelled with the relevant number. The replication of DNA begins at a special site with the formation of a'. single strands. These strands then act as 2 . for the synthesis of new DNA. One strand is called the 3. called the 4.. During DNA replication 5. bases along the two strands. leading to the molecule forming into two .... and the other is .align with complementary
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