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- Assume that you have an "X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. a) Prepare a standard curve by using the absorbance of known concentrations b) Calculate the concentration of unknown. Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220 0.050 0.440 0.100 0.760 0.150 1.250 0.200 1,460 unknown 0,640Refraction is directly related to concentration. true or false? O True O FalseTRUE OR FALSE A Spectroscopic Binary is revealed by alternating red and blue shifts in its spectrum.
- a) A colorimeter is a type of spectrophotometer which is used to measures the light transmitted through a sample at a specific wavelength. True or False. b) As the cell path length shortens, the percent transmittance increases. True or false. c) As the absorbance value increase, the transmittance decrease. True or false. d) Ultraviolet-visible spectroscopy measures the absorption of plane-polarized light to determine the three-dimensional structure of a compound. True or false.What absorbance corresponds to 1% transmittance? To 50% transmittance?Chemical dyes are used to increase resolution of objects. True False
- Question 4 What is the correct order of the components in a UV-Visible spectrophotometer? A) Sample Light source →→ Monochromator→ Photodetector B Light Source →→→ Sample → Monochromator→ Photodetector C Light source → Sample Photodetector → Monochromator D Light Source →→→ Monochromator→ Sample Photodetector ContinueA student generated the following calibration curve using Allura red. They measured the absorbance of a sample containing an unknown concentration of Allura red at the same wavelength. The absorbance of the unknown sample was equal to 0.5. Use the calibration curve to estimate the concentration of the dye in the sample.When an electron is excited from a lower energy state to a higher state, this is known as Emission Diffraction Absorption Refraction