Assume that you have an "X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. a) Prepare a standard curve by using the absorbance of known concentrations b) Calculate the concentration of unknown. Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220 0.050 0.440 0.100 0.760 0.150 1.250 0.200 1,460 unknown 0,640

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Assume that you have an "X" solution that you do not know its concentration. But you have
other X solutions with known concentrations and you know X solution absorbs maximum light
at 575 nm. To calculate the unknown solution concentration, you have done some
spectrophotometric measurements and obtained data given below.
a)
Prepare a standard curve by using the absorbance of known concentrations
b)
Calculate the concentration of unknown.
Table 1: Absorbance of X solution (575 nm)
Concentration (mg/ml)
Absorbance
0.025
0.220
0.050
0.440
0.100
0.760
0.150
1.250
0.200
1,460
unknown
0,640
Transcribed Image Text:Assume that you have an "X" solution that you do not know its concentration. But you have other X solutions with known concentrations and you know X solution absorbs maximum light at 575 nm. To calculate the unknown solution concentration, you have done some spectrophotometric measurements and obtained data given below. a) Prepare a standard curve by using the absorbance of known concentrations b) Calculate the concentration of unknown. Table 1: Absorbance of X solution (575 nm) Concentration (mg/ml) Absorbance 0.025 0.220 0.050 0.440 0.100 0.760 0.150 1.250 0.200 1,460 unknown 0,640
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