The progress of a hypothetical reaction: A + B C+DE, was monitored using paper chromatography. Compounds A and B were mixed and a sample of the solution was spotted every five minutes. Same stationary phase and mobile phase were used throughout the experiment. The figure below shows chromatograms at different time points. Lane R corresponds to the migration of standards A, B, C, D, and E. R O min 5 mins 10 mins 15 mins 20 mins 25 mins 30 mins A. Calculate the retention factor of A, B, C, D, and E given that the order of polarity is D > E> A >C> B. A = B = C D = E = II II

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The progress of a hypothetical reaction: A + B →C+D E, was monitored using paper
chromatography. Compounds A and B were mixed and a sample of the solution was
spotted every five minutes. Same stationary phase and mobile phase were used
throughout the experiment. The figure below shows chromatograms at different time
points. Lane R corresponds to the migration of standards A, B, C, D, and E.
R
O min
5 mins
10 mins
15 mins
20 mins 25 mins 30 mins
A. Calculate the retention factor of A, B, C, D, and E given that the order of polarity is D >
E> A>C> B.
A =
B =
C
D =
E =
II II
Transcribed Image Text:The progress of a hypothetical reaction: A + B →C+D E, was monitored using paper chromatography. Compounds A and B were mixed and a sample of the solution was spotted every five minutes. Same stationary phase and mobile phase were used throughout the experiment. The figure below shows chromatograms at different time points. Lane R corresponds to the migration of standards A, B, C, D, and E. R O min 5 mins 10 mins 15 mins 20 mins 25 mins 30 mins A. Calculate the retention factor of A, B, C, D, and E given that the order of polarity is D > E> A>C> B. A = B = C D = E = II II
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