The number of DNA molecules produced after 30 cycles of PCR, starting with a single molecule, is approximately Choose an answer below: 1 billion. 230. 302. a and b, but not c a and c, but not b
The number of DNA molecules produced after 30 cycles of PCR, starting with a single molecule, is approximately
Choose an answer below:
230.
302.
Polymerase Chain Reaction (PCR) is a gene or DNA amplifying instrument widely used in biotechnology. A huge amount of amplified product can be obtained from nanograms of the initial template. The PCR sample mixture consists of a template, primers, Taq polymerase, master mix or reagent, and dNTP mix (dATP, dCTP, dGTP, and dTTP).
The PCR consist of three temperature-dependent steps
- Denaturation: the sample is heated for one minute. The double stranded DNA (dsDNA) template strand denatures and from single strands.
- Annealing: the sample is cooled for two minutes, and the primer gets attached to the single stranded DNA template.
- Extension: the primer is allowed to extended or polymerized using deoxynucleotides (dNTP) in the mixture for three minutes to form new dsDNA.
The process is repeated to obtained amplified copies of the desired DNA or gene. The number of copies obtained is 2n times in n cycles.
thus, ‘a and b, but not c’ is the correct option
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