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QUESTION 16 Consider each of the three calculation problems related to DNA extraction. A. What is the concentration of a DNA extract which was diluted 1:10 and had an A260 reading of 0.40? B. You elute the DNA sample above by adding 180 μl buffer. What is the total DNA yield, in ug? c. The A280 reading of the DNA sample is 0.24, and the A230 reading is 0.25. How pure is your DNA sample?

QUESTION 16 Consider each of the three calculation problems related to DNA extraction. A. What is the concentration of a DNA extract which was diluted 1:10 and had an A260 reading of 0.40? B. You elute the DNA sample above by adding 180 μl buffer. What is the total DNA yield, in ug? c. The A280 reading of the DNA sample is 0.24, and the A230 reading is 0.25. How pure is your DNA sample?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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QUESTION 16 Consider each of the three calculation problems related to DNA extraction. A. What is the concentration of a DNA extract which was diluted 1:10 and had an A260 reading of 0.40? B. You elute the DNA sample above by adding 180 μl buffer. What is the total DNA yield, in ug? c. The A280 reading of the DNA sample is 0.24, and the A230 reading is 0.25. How pure is your DNA sample?
Transcribed Image Text:QUESTION 16 Consider each of the three calculation problems related to DNA extraction. A. What is the concentration of a DNA extract which was diluted 1:10 and had an A260 reading of 0.40? B. You elute the DNA sample above by adding 180 μl buffer. What is the total DNA yield, in ug? c. The A280 reading of the DNA sample is 0.24, and the A230 reading is 0.25. How pure is your DNA sample?
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