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The main of objective of using 13-Line streaking method is to get:
A-Contaminated cultures
B-None of the above
C-Overlapped colonies
D-Separated colonies
Step by step
Solved in 2 steps
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedWhat are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage DisadvantageWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle
- 3. A bacterial culture was diluted and results from duplicate plates were obtained as indicated below. What was the number of colony forming units/mL of the original culture? Dilution used for plating 10-2 10-3 104 10-5 10⁰ 10-7 10-8 4. 5. Amount plated 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL 0.1 mL Colony counts after incubation (Results from duplicate plates) Too many to count Too many to count 341 413 99 175 27 : 29 7:2 0:0 Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar. After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gram sample of hamburger? Devise a serial dilution scheme to prepare a 10-5 dilution on a plate using the least number of…You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?Describe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).
- A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative StainWhy is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: O So that cells are not clumped and don't entrap stain creating erroneus results So that no contaminants are introduced onto the slide by being entrapped in clumps OSo that the cells are spread out enough that cell morphology can be discerned OSo that the cells are spread out enough that the arrangement can be observed O So that there are small groups of cells clumped together to make them visible Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreader
- Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could’ve caused this? Explain.PCR tests are becoming more prevalent as a standard for testing for bacterial or viral disease. Describe how PCR would be used in place of a growth culture for Strep throat and why this would be advantageous.Which of the following is not meant to reduce contamination of yoru sample? avoiding hand contact with the speciman none of these heating the inoculation loop until red heat fixation of the speciman flaming the lip of a test tube culture