The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a single polycistronic mRNA. The new lac strain of Escherichia coli are as shown below: P+O+Z+Y+A+// P+O+Z+Y+A¯ (i) Illustrate how lactose induces the gene expression in lac 1 $// I-. (ii) Explain the function of the lacP and laco in the bacterial operon.

Q: In the lac operon system, do the inducers act at the transcription or translation level? Explain why…

A: The lactose operon (lac operon) consists of a regulatory gene (i-gene) and three structural genes -…

Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…

A: ii) LacP is called the promoter gene of lac operon.

Q: Diagram the lactose operon in E. coli with the properorder of the elements lacI, lacO, lacY, and…

A: An operon is a group of genes that are expressed together under a common promoter and terminator.…

Q: For the following questions, describe the expression levels of the structural genes in the Trp…

A: Tryptophan is an essential amino acid, and the trp operon is a genetic regulatory system that can be…

Q: The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a…

A: Lac operon has *3 structural genes- lacZ, lacY, lacA *promoter, *operator, *terminator, *regulator.

Q: Explain why large amounts of transcript result when a bacterium with a lac Operon is in a medium…

A: Transcription is the process by which RNA is synthesized from DNA. The messenger RNA or mRNA…

Q: The trp operon, found in E. coli, is a group of genes that encode biosynthetic enzymes for the amino…

A: When tryptophan levels are low tryptophan operon  is induced to transcribe structural genes so that…

Q: 5' 3' ORF1 ORF2 ORF3 ORF4 ORF5 1. Above are pictured 2 operons, one that includes ORF1 and OFR2 and…

A: The production of RNA from DNA is known as transcription that occurs within the cytoplasm of…

Q: IPTG can be used in the laboratory as a synthetic inducer of the lac operon, instead of lactose.…

A: Lactose operon is an example of inducible operon in which the expression of downstream genes will be…

Q: Strain LOLZ has a mutation in the lacI gene that results in a lacI protein that cannot bind…

A: Lac operon is the segment of DNA which is responsible for breaking down of lactose into glucose and…

Q: The highest level of transcription of the E. coli lac operon occurs when CAP (+cAMP) is bound to the…

A: The lac operon in E. coli is a system of genes that regulates the metabolism of lactose, a sugar.…

Q: The trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the…

A: trp operon is a repressor operon found in E. coli bacteria, which consists of a group of genes that…

Q: Considering the Lac operon in E. coli, and chose what the expression of Lac Y would be in:  I) E.…

A: Lac l is the gene  which synthesizes repressor.Actually repressor is a protein that binds to the…

Q: 1 [Choose ] Choose Promoter Operator Regulatory Gene Active Repressor Protein Structural Genes 3…

A: Lac operon is basically a mechanism essential in the metabolism in the E.coli bacteria.

Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

A: Inactivating mutations, also known as loss-of-function mutations, cause the gene product to have…

Q: P+OcZ-Y+A+// P¬O+Z+Y+A+ i) The lac operon consists of three structural genes, lacZ, lacY and lacA.…

A: The lactose operon (also known as the lac operon) is a set of genes that are specific for the uptake…

Q: . Partial diploid forms of E. coli were created, each of which contained a com- plete lactose operon…

A: Lac operon is composed of lacI, P, lacO, lacZ, lacY and lacA genes. Regulatory genes are lacI, P and…

Q: Predict the growth/color pattern you'd expect for constitutive mutants for the lac operon. Table 1:…

A:

Q: If a wild-type (normal, NOTmutated) E. coli strain is grown in a medium: a. without lactose or…

A: The lac operon or the lactose operon is a mechanism that is involved in the metabolism and transport…

Q: 12) The top operon in E. coli is a group of genes that control the biosynthesis of tryptophan.…

A: Step 1: TRP operon in E. coli is a classical case of gene regulation in prokaryotes' It regulates…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: An E. coli lac- mutant (a mutant that cannot grow in the medium containing lactose as only carbon…

A: Operon, is a bacterial and viral genetic regulation system in which genes coding for functionally…

Q: Imagine that a new strain of E. coli is discovered, and it contains the following novel genetic…

A: The lac operon is an operon, which is a collection of genes with a single promoter. The genes in the…

Q: Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor.…

A: The lac operon is an operon, or group of genes with a single promoter.The genes in the operon encode…

Q: The IMD2 promoter contains three upstream transcription start sites (TSS) that are utilized under…

A: Inosine monophosphate dehydrogenase (IMD2) is a key enzyme in the purine biosynthesis pathway that…

Q: 15a) Most amino acid biosynthetic operons in prokaryotes have a structure similar to that observed…

A: The tryptophan operon consists of five structural genes: trpE, trpD, trpC, trpB, trpA that codes for…

Q: 6) The C-terminal domain (CTD) of yeast RNA polymerase II (RPB) subunit RPB1 contains the repeating…

A: RNA polymerase: it is the enzyme involved in transcription of genetic material, DNA.

Q: Strain HK180 has lac operon with a nonfunctional lacP. Otherwise all other parts of the operon are…

A: The promoter is a DNA sequence located upstream of the target gene to be transcribed, to which RNA…

Q: The symbols A, B, C, D, E represent all the components of the lac operon, excluding LacA. The…

A: The lac operon regulates gene expression in lactose metabolism in E. coli. It is composed of a…

Q: For the trp operon, determine whether genes will be expressed (on) or silenced (off) given the…

A: Gene is a basic unit of hereditary information or data that fills a fixed position on a chromosome.…

Q: Calculate the concentration of lac repressor, assuming that one molecule is present per cell. Assume…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Q: Given the following mutant and conditions, predict the expression of the lacZ gene (no expression,…

A: Introduction An operon is a functional unit of DNA (Deoxyribonucleic acid) containing a cluster of…

Q: Explain how elements of the lac operon are exploited to regulate recombinant protein production in…

A: Introduction Three structural genes, as well as a promoter, a terminator, a regulator, and an…

Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…

A: The operon model is applicable in bacterial gene regulation and also applicable in some lower…

Q: The lac genotypes are as shown below: P+OcZ-Y+A+// P¬O+Z+Y+A+ (i) The lac operon consists of three…

A: Lac operon refers to the set of genes responsible for uptake and metabolism of lactose. Three…

Q: In a bacterial cell with the genotype I-: P+: O+: Z+: Y+: A+, which of the following expression…

A: The lac operon in bacterial cells plays a pivotal role in the regulation of lactose metabolism.…

Q: For each of the following conditions in the Lac Operon, state whether transcription will occur or…

A: The lac operon of E. coli contains genes responsible  in lactose metabolism. It is  expressed only…

Q: (iii) What is the function of the promoter in the bacterial operon?

A: The lactose operon (also abbreviated as the lac operon) is a group of genes present in E. coli and…

Gene Interactions

When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.

Gene Expression

Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.